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Human Golgi reassembly-stacking protein 1 ELISA Kit | GORASP1 elisa kit

Human Golgi reassembly-stacking protein 1 (GORASP1) ELISA Kit

Gene Names
GORASP1; P65; GOLPH5; GRASP65
Reactivity
Human
Synonyms
Golgi reassembly-stacking protein 1; Human Golgi reassembly-stacking protein 1 (GORASP1) ELISA Kit; GORASP1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human GORASP1. No significant cross-reactivity or interference between Human GORASP1 and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for GORASP1 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GORASP1 in samples. An antibody specific for GORASP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGORASP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GORASP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GORASP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,237 Da
NCBI Official Full Name
Golgi reassembly-stacking protein 1 isoform 2
NCBI Official Synonym Full Names
golgi reassembly stacking protein 1
NCBI Official Symbol
GORASP1
NCBI Official Synonym Symbols
P65; GOLPH5; GRASP65
NCBI Protein Information
Golgi reassembly-stacking protein 1
UniProt Protein Name
Golgi reassembly-stacking protein 1
UniProt Gene Name
GORASP1
UniProt Synonym Gene Names
GOLPH5; GRASP65; GOLPH5; GRASP65

NCBI Description

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

GRASP65: Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes. Belongs to the GORASP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 3p22.2

Cellular Component: endoplasmic reticulum-Golgi intermediate compartment membrane; Golgi apparatus; Golgi membrane

Molecular Function: protein binding

Biological Process: COPII vesicle coating; ER to Golgi vesicle-mediated transport; Golgi organization and biogenesis; negative regulation of dendrite morphogenesis; positive regulation of ubiquitin protein ligase activity; protein N-linked glycosylation; protein transport

Research Articles on GORASP1

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Product Notes

The Human GORASP1 gorasp1 (Catalog #AAA280150) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280150 ELISA Kit recognizes Human GORASP1. It is sometimes possible for the material contained within the vial of "Golgi reassembly-stacking protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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