Bovine Apoptosis Regulator BAX (BAX) ELISA Kit | BAX elisa kit
Bovine Apoptosis Regulator BAX (BAX) ELISA Kit
Reactivity
Bovine
Synonyms
Apoptosis Regulator BAX (BAX); Bovine Apoptosis Regulator BAX (BAX) ELISA Kit; BCL2L4; apoptosis regulator BAX; BAX elisa kit
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of Bovine BAX. No significant cross-reactivity or interference between Bovine BAX and analogues was observed.
Sequence Length
192
Assay Type
Quantitative Sandwich
Samples
Serum, Plasma, Other biological fluids
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C. Please refer to Instruction Manual.
Related Product Information for BAX elisa kit
This assay employs a two-site sandwich ELISA to quantitate BAX in samples. An antibody specific for BAX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BAX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BAX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BAX bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
apoptosis regulator BAX
NCBI Official Synonym Full Names
BCL2 associated X, apoptosis regulator
NCBI Official Symbol
BAX
NCBI Protein Information
apoptosis regulator BAX
UniProt Protein Name
Apoptosis regulator BAX
Protein Family
UniProt Gene Name
BAX
Uniprot Description
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. BAX deficiency leads to lymphoid hyperplasia and male sterility, because of the cessation of sperm production (). Interacts (via a C-terminal 33 residues) with NOL3 (via CARD domain); inhibits BAX activation and translocation and consequently cytochrome c release from mitochondria ().
Research Articles on BAX
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Product Notes
The Bovine BAX bax (Catalog #AAA2801447) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2801447 ELISA Kit recognizes Bovine BAX. It is sometimes possible for the material contained within the vial of "Apoptosis Regulator BAX (BAX), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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