Human Myosin-10 (MYH10) ELISA Kit | MYH10 elisa kit

Human Myosin-10 (MYH10) ELISA Kit

Cat. Num. AAA2801360

• Gene Names: MYH10; NMMHCB; NMMHC-IIB
• Reactivity: Human
• Synonyms: Myosin-10 (MYH10); Human Myosin-10 (MYH10) ELISA Kit; MGC134913; MGC134914; NMMHCB; cellular myosin heavy chain; type B type B; myosin heavy chain; nonmuscle type B; myosin; heavy polypeptide 10; non-muscle; near to the ATP binding region; nonmuscle myosin; MYH10 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MYH10. No significant cross-reactivity or interference between Human MYH10 and analogues was observed.
• Sequence Length: 1976

• Assay Type: Detection Method: Sandwich; Analysis Method: Quantitive
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: 0.055 ng/mL
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MYH10 elisa kit

Test Principle: This assay employs a two-site sandwich ELISA to quantitate MYH10 in samples. An antibody specific for MYH10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYH10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYH10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYH10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Overview: Myh10 was abundantly expressed in mouse brain and testis. It was also expressed in heart, lung, liver, and kidney, but not in skeletal muscle or spleen. Actin-activated MgATPase activity was decreased in MYH10 with either an asn97-to-lysine substitution, which is homologous to the N93K mutation in MYH9 that causes May-Hegglin anomaly, or an arg709-to-cysteine substitution, which causes developmental defects in brain and heart when present in mouse Myh10. The ability of MYH10 heavy meromyosin to support the movement of actin filaments over an MYH10-coated surface was reduced in heavy meromyosin with the N97K mutation and eliminated with the R709C mutation. Kinetic analysis indicated that the R709C mutation resulted in extremely tight affinity between MYH10 heavy meromyosin and ADP, reducing the rate of ADP release.

NCBI and Uniprot Product Information

• NCBI GI #: 215274129
• NCBI GeneID: 4628
• UniProt Accession #: P35580; Q12989; Q149N3; Q149N4; Q16087; Q4LE45; Q6PK16; Q9BWG0; B2RWP9; D3DTS1; F8VTL3
• Molecular Weight: 230,779 Da
• NCBI Official Full Name: Myosin-10
• NCBI Official Synonym Full Names: myosin heavy chain 10
• NCBI Official Symbol: MYH10
• NCBI Official Synonym Symbols: NMMHCB; NMMHC-IIB
• NCBI Protein Information: myosin-10
• UniProt Protein Name: Myosin-10
• Protein Family: Myosin
• UniProt Gene Name: MYH10
• UniProt Synonym Gene Names: NMMHC-B; NMMHC II-b; NMMHC-IIB

NCBI Description

This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9.