Mouse Guanylate Binding Protein 2, Interferon Inducible (GBP2) ELISA Kit | GBP2 elisa kit

Mouse Guanylate Binding Protein 2, Interferon Inducible (GBP2)(Sandwich ELISA) ELISA Kit

Cat. Num. AAA2709706

• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Guanylate Binding Protein 2; Interferon Inducible (GBP2); Mouse Guanylate Binding Protein 2; Interferon Inducible (GBP2)(Sandwich ELISA) ELISA Kit; ELISA Kit for Guanylate Binding Protein 2; GBP2/Guanylate Binding Protein 2; Interferon Inducible; GTP-binding protein 2; Guanine nucleotide-binding protein 2; GBP2 elisa kit

• Reactivity: Mouse
• Specificity: Reactive with Mouse Guanylate Binding Protein 2, Interferon Inducible (GBP2); This assay has high sensitivity and excellent specificity for detection of Guanylate Binding Protein 2, Interferon Inducible (GBP2). No significant cross-reactivity or interference between Guanylate Binding Protein 2, Interferon Inducible (GBP2) and analogues was observed.

• Applicable Applications for GBP2 elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Assay Type: Sandwich ELISA
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Method: Double-antibody Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.061ng/mL
• Assay Length: 3 h
• Sample Size: 100ul (Please refer to the manual for the specific data.)
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Guanylate Binding Protein 2, Interferon Inducible (GBP2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Guanylate Binding Protein 2, Interferon Inducible (GBP2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C; 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C; 4. Aspirate and wash 3 times; 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 6. Aspirate and wash 5 times; 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C; 8. Add 50ul Stop Solution. Read at 450nm immediately.
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 3 (GAL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galectin 3 (GAL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galectin 3 (GAL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Galectin 3 (GAL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: Store at 4 degree C for 1 month/ -20 degree C for long-term (within shelf life).; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for GBP2 elisa kit

Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 6753950
• NCBI GeneID: 14469
• NCBI Accession #: NP_034390.1
• NCBI GenBank Nucleotide #: NM_010260.1
• UniProt Accession #: Q9Z0E6; Q4FK03; Q8CIC6; Q921N2; Q9R1I0
• Molecular Weight: 66,739 Da
• NCBI Official Full Name: interferon-induced guanylate-binding protein 2
• NCBI Official Synonym Full Names: guanylate binding protein 2
• NCBI Official Symbol: Gbp2
• NCBI Protein Information: interferon-induced guanylate-binding protein 2
• UniProt Protein Name: Interferon-induced guanylate-binding protein 2
• Protein Family: Guanylate-binding protein
• UniProt Gene Name: Gbp2
• UniProt Synonym Gene Names: GBP-2; mGBP-2; mGBP2
• UniProt Entry Name: GBP2_MOUSE

Uniprot Description

GBP2: a member of the family of large GTP-binding proteins (GBPs) that is inducible by interferon. Possesses a high GTP hydrolysis activity. GBPs are the most abundant cellular proteins expressed in response to interferon-gamma (IFN-gamma). IFN-gamma, tumor necrosis factor-alpha (TNF-alpha), and interleukin-1beta (IL-1beta) strongly induce the expression of GBP-1, -2, and -3.

Protein type: Hydrolase; Vesicle

Cellular Component: Golgi apparatus; membrane; cytoplasm; cytoplasmic vesicle; nucleus

Molecular Function: GTPase activity; GTP binding; nucleotide binding

Biological Process: defense response to Gram-positive bacterium; adhesion to host; defense response to protozoan