Typical Testing Data/Standard Curve (for reference only)
Human Regenerating Islet Derived Protein 3 Alpha (REG3a) ELISA Kit | REG3a elisa kit
Human Regenerating Islet Derived Protein 3 Alpha (REG3a) Wide-range ELISA Kit
Gene Names
REG3A; HIP; PAP; PAP1; REG3; INGAP; PAP-H; PBCGF; HIP/PAP; REG-III
Reactivity
Human
Applications
ELISA
Synonyms
Regenerating Islet Derived Protein 3 Alpha (REG3a); Human Regenerating Islet Derived Protein 3 Alpha (REG3a) Wide-range ELISA Kit; Regenerating Islet Derived Protein 3 Alpha; HIP; INGAP; PAP-H; PAP1; PBCGF; REG-III; REG3; Pancreatitis-Associated Protein; Hepatointestinal pancreatic protein; Human proislet peptide; Pancreatitis-associated protein 1; REG3a elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a).
No significant cross-reactivity or interference between Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a) and analogues was observed.
No significant cross-reactivity or interference between Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a) and analogues was observed.
Sequence Length
175
Applicable Applications for REG3a elisa kit
ELISA (EIA)
Assay Type
Double-antibody Sandwich
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
3.12-200ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 1.14ng/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Wide-range Regenerating Islet Derived Protein 3 Alpha (REG3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
regenerating islet-derived protein 3-alpha
NCBI Official Synonym Full Names
regenerating family member 3 alpha
NCBI Official Symbol
REG3A
NCBI Official Synonym Symbols
HIP; PAP; PAP1; REG3; INGAP; PAP-H; PBCGF; HIP/PAP; REG-III
NCBI Protein Information
regenerating islet-derived protein 3-alpha
UniProt Protein Name
Regenerating islet-derived protein 3-alpha
Protein Family
UniProt Gene Name
REG3A
UniProt Synonym Gene Names
HIP; PAP; PAP1; REG-3-alpha; HIP/PAP
UniProt Entry Name
REG3A_HUMAN
NCBI Description
This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The mature protein also functions as an antimicrobial protein with antibacterial activity. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Nov 2014]
Uniprot Description
REG3A: Might be a stress protein involved in the control of bacterial proliferation.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2p12
Cellular Component: extracellular space; cytoplasm
Molecular Function: carbohydrate binding
Biological Process: heterophilic cell adhesion; cell proliferation; multicellular organismal development; negative regulation of keratinocyte differentiation; acute-phase response
Research Articles on REG3a
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Product Notes
The Human REG3a reg3a (Catalog #AAA2709321) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2709321 ELISA Kit recognizes Human REG3a. AAA Biotech's Regenerating Islet Derived Protein 3 Alpha (REG3a) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the REG3a reg3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Regenerating Islet Derived Protein 3 Alpha (REG3a), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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