Mouse Interleukin 1 Family, Member 9 (IL1F9) CLIA Kit | IL1F9 clia kit

Mouse Interleukin 1 Family, Member 9 (IL1F9) CLIA Kit

Cat. Num. AAA2709223

• Gene Names: Il1f9; Il36g
• Reactivity: Mouse
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Interleukin 1 Family; Member 9 (IL1F9); Mouse Interleukin 1 Family; Member 9 (IL1F9) CLIA Kit; Member 9; IL-1F9; IL36g; IL-1H1; IL-1RP2; IL1E; IL1H1; IL1RP2; Interleukin 36; Gamma; Interleukin-1 Homolog 1; Interleukin-1 epsilon; Interleukin 1-Related Protein 2; IL1F9 clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Family, Member 9 (IL1F9).; No significant cross-reactivity or interference between Interleukin 1 Family, Member 9 (IL1F9) and analogues was observed.
• Sequence Length: 164

• Applicable Applications for IL1F9 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 1.37-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.46pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Family, Member 9 (IL1F9) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Family, Member 9 (IL1F9) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Family, Member 9 (IL1F9). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 1 Family, Member 9 (IL1F9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 1 Family, Member 9 (IL1F9) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 25008588
• NCBI GeneID: 215257
• UniProt Accession #: Q8R460
• NCBI Official Full Name: Interleukin-36 gamma
• NCBI Official Synonym Full Names: interleukin 1 family, member 9
• NCBI Official Symbol: Il1f9
• NCBI Official Synonym Symbols: Il36g
• NCBI Protein Information: interleukin-36 gamma
• UniProt Protein Name: Interleukin-36 gamma
• UniProt Gene Name: Il36g
• UniProt Synonym Gene Names: Il1f9; IL-1F9
• UniProt Entry Name: IL36G_MOUSE

Uniprot Description

Functions as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2/IL1RL2. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP (). Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. May play a role in proinflammatory responses during particular neutrophilic airway inflammation. May be involved in the innate immune response to fungal pathogens. Induces the production of proinflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4+ T-cells and splenocytes.