Human Melanocortin 5 Receptor (MC5R) CLIA Kit | MC5R clia kit

Human Melanocortin 5 Receptor (MC5R) CLIA Kit

Cat. Num. AAA2709164

• Gene Names: MC5R; MC2
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Melanocortin 5 Receptor (MC5R); Human Melanocortin 5 Receptor (MC5R) CLIA Kit; Melanocortin 5 Receptor; MC-2; MC5R clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Melanocortin 5 Receptor (MC5R).; No significant cross-reactivity or interference between Melanocortin 5 Receptor (MC5R) and analogues was observed.
• Sequence Length: 325

• Applicable Applications for MC5R clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and other biological fluids
• Detection Range: 13.7-10,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 5.5pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanocortin 5 Receptor (MC5R) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanocortin 5 Receptor (MC5R) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Melanocortin 5 Receptor (MC5R). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Melanocortin 5 Receptor (MC5R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Melanocortin 5 Receptor (MC5R) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 5174535
• NCBI GeneID: 4161
• NCBI Accession #: NP_005904.1
• NCBI GenBank Nucleotide #: NP_005904.1
• UniProt Accession #: P33032
• NCBI Official Full Name: melanocortin receptor 5
• NCBI Official Synonym Full Names: melanocortin 5 receptor
• NCBI Official Symbol: MC5R
• NCBI Official Synonym Symbols: MC2
• NCBI Protein Information: melanocortin receptor 5
• UniProt Protein Name: Melanocortin receptor 5
• Protein Family: Melanocortin receptor
• UniProt Gene Name: MC5R
• UniProt Synonym Gene Names: MC5-R
• UniProt Entry Name: MC5R_HUMAN

NCBI Description

This gene encodes a member of the seven-pass transmembrane G protein-coupled melanocortin receptor protein family that stimulate cAMP signal transduction. The encoded protein is a receptor for melanocyte-stimulating hormone and adrenocorticotropic hormone and is suggested to play a role in sebum generation. [provided by RefSeq, Jun 2010]

Uniprot Description

MC5R: Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. This receptor is a possible mediator of the immunomodulation properties of melanocortins. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 18p11.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; melanocortin receptor activity; hormone binding

Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; positive regulation of cAMP biosynthetic process