Rat Plasminogen Activator Inhibitor 1 (PAI1) CLIA Kit | PAI1 clia kit

Rat Plasminogen Activator Inhibitor 1 (PAI1) CLIA Kit

Cat. Num. AAA2708780

• Gene Names: Serpine1; Pai1; PAI1A; Planh; Pai1aa; RATPAI1A
• Reactivity: Rat
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Plasminogen Activator Inhibitor 1 (PAI1); Rat Plasminogen Activator Inhibitor 1 (PAI1) CLIA Kit; Plasminogen Activator Inhibitor 1; SERPINE1; PLANH1; Serpin Peptidase Inhibitor Clade E Member 1; Nexin; Plasminogen Activator Inhibitor Type 1 Serpin E1; Endothelial plasminogen activator inhibitor; PAI1 clia kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Plasminogen Activator Inhibitor 1 (PAI1).; No significant cross-reactivity or interference between Plasminogen Activator Inhibitor 1 (PAI1) and analogues was observed.
• Sequence Length: 402

• Applicable Applications for PAI1 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Plasma
• Detection Range: 27.4-20,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 11.5pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plasminogen Activator Inhibitor 1 (PAI1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plasminogen Activator Inhibitor 1 (PAI1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Plasminogen Activator Inhibitor 1 (PAI1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Plasminogen Activator Inhibitor 1 (PAI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Plasminogen Activator Inhibitor 1 (PAI1) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 6981332
• NCBI GeneID: 24617
• NCBI Accession #: NP_036752.1
• NCBI GenBank Nucleotide #: NP_036752.1
• UniProt Accession #: P20961
• NCBI Official Full Name: plasminogen activator inhibitor 1
• NCBI Official Synonym Full Names: serpin family E member 1
• NCBI Official Symbol: Serpine1
• NCBI Official Synonym Symbols: Pai1; PAI1A; Planh; Pai1aa; RATPAI1A
• NCBI Protein Information: plasminogen activator inhibitor 1
• UniProt Protein Name: Plasminogen activator inhibitor 1
• UniProt Gene Name: Serpine1
• UniProt Synonym Gene Names: Pai1; Planh1; PAI; PAI-1
• UniProt Entry Name: PAI1_RAT

NCBI Description

mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator; may promote neuronal survival [RGD, Feb 2006]

Uniprot Description

SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protease binding; receptor binding

Biological Process: positive regulation of blood coagulation; wound healing; response to lipopolysaccharide; positive regulation of coagulation; decidualization; negative regulation of smooth muscle cell migration; glucose homeostasis; defense response to Gram-negative bacterium; mast cell activation; positive regulation of receptor-mediated endocytosis; positive regulation of interleukin-8 production; positive regulation of fibroblast proliferation; negative regulation of fibrinolysis; tissue regeneration; angiogenesis; regulation of receptor activity; chronological cell aging; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; female gonad development; negative regulation of proteolysis; positive regulation of keratinocyte migration; regulation of angiogenesis; regulation of cell proliferation; response to starvation; response to reactive oxygen species; positive regulation of angiogenesis; response to hyperoxia; cellular response to insulin stimulus; response to estrogen stimulus; response to cytokine stimulus; positive regulation of inflammatory response