Mouse X-Ray Repair Cross Complementing 6 (XRCC6) CLIA Kit | XRCC6 clia kit

Mouse X-Ray Repair Cross Complementing 6 (XRCC6) CLIA Kit

Cat. Num. AAA2708747

• Gene Names: Xrcc6; Ku70; 70kDa; G22p1
• Reactivity: Mouse
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: X-Ray Repair Cross Complementing 6 (XRCC6); Mouse X-Ray Repair Cross Complementing 6 (XRCC6) CLIA Kit; X-Ray Repair Cross Complementing 6; Ku70; CTC75; CTCBF; G22P1; ML8; TLAA; Lupus Ku Autoantigen Protein P70; ATP-Dependent DNA Helicase 2 Subunit 1; CTC box-binding factor 75 kDa; Thyroid-lupus autoantigen; XRCC6 clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of X-Ray Repair Cross Complementing 6 (XRCC6).; No significant cross-reactivity or interference between X-Ray Repair Cross Complementing 6 (XRCC6) and analogues was observed.
• Sequence Length: 608

• Applicable Applications for XRCC6 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and other biological fluids
• Detection Range: 13.7-10,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 4.9pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level X-Ray Repair Cross Complementing 6 (XRCC6) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level X-Ray Repair Cross Complementing 6 (XRCC6) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to X-Ray Repair Cross Complementing 6 (XRCC6). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to X-Ray Repair Cross Complementing 6 (XRCC6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the X-Ray Repair Cross Complementing 6 (XRCC6) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 145587104
• NCBI GeneID: 14375
• NCBI Accession #: NP_034377.2
• NCBI GenBank Nucleotide #: NP_034377.2
• UniProt Accession #: P23475
• NCBI Official Full Name: X-ray repair cross-complementing protein 6
• NCBI Official Synonym Full Names: X-ray repair complementing defective repair in Chinese hamster cells 6
• NCBI Official Symbol: Xrcc6
• NCBI Official Synonym Symbols: Ku70; 70kDa; G22p1
• NCBI Protein Information: X-ray repair cross-complementing protein 6
• UniProt Protein Name: X-ray repair cross-complementing protein 6
• Protein Family: X-ray repair cross-complementing protein
• UniProt Gene Name: Xrcc6
• UniProt Synonym Gene Names: G22p1; Ku70; 5'-dRP/AP lyase Ku70; CTC75; CTCBF; Ku70

Uniprot Description

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.