Human Interferon Alpha 2 (IFNa2) CLIA Kit | IFNa2 clia kit

Human Interferon Alpha 2 (IFNa2) CLIA Kit

Cat. Num. AAA2708728

• Gene Names: IFNA2; IFNA; IFNA2B; leIF A; IFN-alphaA; IFN-alpha-2
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Interferon Alpha 2 (IFNa2); Human Interferon Alpha 2 (IFNa2) CLIA Kit; Interferon Alpha 2; IFNA2A; IFNA2B; IFNA2C; LeIF A; Alpha-2a Interferon; Interferon Alpha 2b; Interferon Alpha A; IFNa2 clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interferon Alpha 2 (IFNa2).; No significant cross-reactivity or interference between Interferon Alpha 2 (IFNa2) and analogues was observed.
• Sequence Length: 188

• Applicable Applications for IFNa2 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 1.37-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.49pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha 2 (IFNa2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha 2 (IFNa2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha 2 (IFNa2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Alpha 2 (IFNa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Alpha 2 (IFNa2) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 11067751
• NCBI GeneID: 3440
• NCBI Accession #: NP_000596.2
• NCBI GenBank Nucleotide #: NP_000596.2
• UniProt Accession #: P01563
• NCBI Official Full Name: interferon alpha-2
• NCBI Official Synonym Full Names: interferon alpha 2
• NCBI Official Symbol: IFNA2
• NCBI Official Synonym Symbols: IFNA; IFNA2B; leIF A; IFN-alphaA; IFN-alpha-2
• NCBI Protein Information: interferon alpha-2
• UniProt Protein Name: Interferon alpha-2
• UniProt Gene Name: IFNA2
• UniProt Synonym Gene Names: IFNA2A; IFNA2B; IFNA2C; IFN-alpha-2; LeIF A
• UniProt Entry Name: IFNA2_HUMAN

NCBI Description

This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded protein is a cytokine produced in response to viral infection. Use of the recombinant form of this protein has been shown to be effective in reducing the symptoms and duration of the common cold. [provided by RefSeq, Jun 2011]