Human Meningioma Expressed Antigen 5 (MGEA5) CLIA Kit | MGEA5 clia kit

Human Meningioma Expressed Antigen 5 (MGEA5) CLIA Kit

Cat. Num. AAA2708584

• Gene Names: OGA; MEA5; MGEA5; NCOAT
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Meningioma Expressed Antigen 5 (MGEA5); Human Meningioma Expressed Antigen 5 (MGEA5) CLIA Kit; Meningioma Expressed Antigen 5; NAGase; MEA5; NCOAT; Hexosaminidase C; N-Acetylhexosaminidase; Hyaluronidase; Meningioma-Expressed Antigen 5; Nuclear Cytoplasmic O-GlcNAcase And Acetyltransferase; MGEA5 clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Meningioma Expressed Antigen 5 (MGEA5).; No significant cross-reactivity or interference between Meningioma Expressed Antigen 5 (MGEA5) and analogues was observed.
• Sequence Length: 863

• Applicable Applications for MGEA5 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Urine
• Detection Range: 0.14-100ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.06ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Meningioma Expressed Antigen 5 (MGEA5) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Meningioma Expressed Antigen 5 (MGEA5) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Meningioma Expressed Antigen 5 (MGEA5). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Meningioma Expressed Antigen 5 (MGEA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Meningioma Expressed Antigen 5 (MGEA5) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 215490056
• NCBI GeneID: 10724
• NCBI Accession #: NP_001135906.1
• NCBI GenBank Nucleotide #: NP_001135906.1
• UniProt Accession #: O60502
• NCBI Official Full Name: protein O-GlcNAcase isoform b
• NCBI Official Synonym Full Names: O-GlcNAcase
• NCBI Official Symbol: OGA
• NCBI Official Synonym Symbols: MEA5; MGEA5; NCOAT
• NCBI Protein Information: protein O-GlcNAcase
• UniProt Protein Name: Protein O-GlcNAcase
• Protein Family: Protein O-GlcNAcase
• UniProt Gene Name: MGEA5
• UniProt Synonym Gene Names: HEXC; KIAA0679; MEA5; NCOAT

NCBI Description

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008]

Uniprot Description

Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).