Mouse Growth Hormone (GH) CLIA Kit | GH clia kit

Mouse Growth Hormone (GH) CLIA Kit

Cat. Num. AAA2708547

• Gene Names: Gh; Gh1; Ghb1
• Reactivity: Mouse
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Growth Hormone (GH); Mouse Growth Hormone (GH) CLIA Kit; Growth Hormone; GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Normal; GH clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Growth Hormone (GH).; No significant cross-reactivity or interference between Growth Hormone (GH) and analogues was observed.
• Sequence Length: 216

• Applicable Applications for GH clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 5.49-4,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 1.95pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone (GH) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone (GH) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Growth Hormone (GH). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Growth Hormone (GH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Growth Hormone (GH) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 6679997
• NCBI GeneID: 14599
• NCBI Accession #: NP_032143.1
• NCBI GenBank Nucleotide #: NP_032143.1
• UniProt Accession #: P06880
• NCBI Official Full Name: somatotropin
• NCBI Official Synonym Full Names: growth hormone
• NCBI Official Symbol: Gh
• NCBI Official Synonym Symbols: Gh1; Ghb1
• NCBI Protein Information: somatotropin
• UniProt Protein Name: Somatotropin
• Protein Family: Envelope glycoprotein
• UniProt Gene Name: Gh1
• UniProt Synonym Gene Names: Gh
• UniProt Entry Name: SOMA_MOUSE

Uniprot Description

GH: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A); also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH. Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B); also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH. Defects in GH1 are the cause of Kowarski syndrome (KWKS); also known as pituitary dwarfism VI. Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2). IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy. Belongs to the somatotropin/prolactin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Hormone

Cellular Component: extracellular space; mitochondrion; extracellular region; plasma membrane; trans-Golgi network; cytosol; nucleus; secretory granule

Molecular Function: growth hormone receptor binding; metal ion binding; hormone activity

Biological Process: response to food; response to light stimulus; positive regulation of neurogenesis; cellular response to insulin stimulus; neuroblast proliferation; positive regulation of multicellular organism growth; glucose transport; regulation of steroid hormone receptor signaling pathway; signal transduction; alveolus development; positive regulation of growth