Rabbit anti-Human, Mouse AMOTL2 Polyclonal Antibody | anti-AMOTL2 antibody

AMOTL2, ID (Leman Coiled-coil Protein, LCCP, Angiomotin-like Protein 2, KIAA0989) (FITC)

Cat. Num. AAA6462670

• Gene Names: AMOTL2; LCCP
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography
• Synonyms: AMOTL2; Polyclonal Antibody; ID (Leman Coiled-coil Protein; LCCP; Angiomotin-like Protein 2; KIAA0989) (FITC); anti-AMOTL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AMOTL2. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-AMOTL2 antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a region near a portion within aa132-161 near the center region of human AMOTL2, conjugated to KLH.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of AMOTL2 Antibody (Center) in A375 cell line lysates (35ug/lane). AMOTL2 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of AMOTL2 Antibody (Center) in mouse liver tissue lysates (35ug/lane). AMOTL2 (arrow) was detected using the purified Pab.)

Related Product Information for anti-AMOTL2 antibody

AMOTL2 is a protein that binds angiostatin, a circulating inhibitor of the formation of new blood vessels (angiogenesis). Angiomotin mediates angiostatin inhibition of endothelial cell migration and tube formation in vitro. The protein encoded by this gene is related to angiomotin and is a member of the motins protein family.

Product Categories/Family for anti-AMOTL2 antibody

Antibodies; Binding Proteins

References

1. Bratt, A., et.al., Gene 298(1), 69-77 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 32484977
• NCBI GeneID: 51421
• NCBI Accession #: NP_057285.3
• NCBI GenBank Nucleotide #: NM_016201.3
• UniProt Accession #: Q9Y2J4; Q53EP1; Q96F99; Q9UKB4; A8K6F1; B7Z5Q1; E9PHW3
• Molecular Weight: 91,817 Da
• NCBI Official Full Name: angiomotin-like protein 2 isoform 2
• NCBI Official Synonym Full Names: angiomotin like 2
• NCBI Official Symbol: AMOTL2
• NCBI Official Synonym Symbols: LCCP
• NCBI Protein Information: angiomotin-like protein 2; Leman coiled-coil protein
• UniProt Protein Name: Angiomotin-like protein 2
• Protein Family: Angiomotin-like protein
• UniProt Gene Name: AMOTL2
• UniProt Synonym Gene Names: KIAA0989; LCCP

NCBI Description

Angiomotin is a protein that binds angiostatin, a circulating inhibitor of the formation of new blood vessels (angiogenesis). Angiomotin mediates angiostatin inhibition of endothelial cell migration and tube formation in vitro. The protein encoded by this gene is related to angiomotin and is a member of the motin protein family. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

AMOTL2: Regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites. Required for proper architecture of actin filaments. Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Participates in angiogenesis. May play a role in the polarity, proliferation and migration of endothelial cells. Selectively promotes FGF-induced MAPK activation through SRC. Belongs to the angiomotin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 3q22.2

Cellular Component: apical plasma membrane; bicellular tight junction; cytosol; recycling endosome

Molecular Function: identical protein binding; protein binding

Biological Process: Wnt signaling pathway