Typical Testing Data/Standard Curve (for reference only)
Human Angiopoietin Like Protein 8 (ANGPTL8) ELISA Kit | ANGPTL8 elisa kit
Human Angiopoietin Like Protein 8 (ANGPTL8) Mini Samples ELISA Kit
Gene Names
ANGPTL8; RIFL; TD26; PRO1185; PVPA599; C19orf80
Reactivity
Human
Applications
ELISA
Synonyms
Angiopoietin Like Protein 8 (ANGPTL8); Human Angiopoietin Like Protein 8 (ANGPTL8) Mini Samples ELISA Kit; Angiopoietin Like Protein 8; RIFL; TD26; C19orf80; Lipasin; Betatrophin; Hepatocellular Carcinoma-Associated Protein TD26; ANGPTL8 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Angiopoietin Like Protein 8 (ANGPTL8).
No significant cross-reactivity or interference between Mini Samples Angiopoietin Like Protein 8 (ANGPTL8) and analogues was observed.
No significant cross-reactivity or interference between Mini Samples Angiopoietin Like Protein 8 (ANGPTL8) and analogues was observed.
Sequence Length
198
Applicable Applications for ANGPTL8 elisa kit
ELISA (EIA)
Assay Type
Double-antibody Sandwich
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.059ng/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Angiopoietin Like Protein 8 (ANGPTL8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Angiopoietin Like Protein 8 (ANGPTL8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Angiopoietin Like Protein 8 (ANGPTL8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Angiopoietin Like Protein 8 (ANGPTL8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Angiopoietin Like Protein 8 (ANGPTL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Angiopoietin Like Protein 8 (ANGPTL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Angiopoietin Like Protein 8 (ANGPTL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
angiopoietin-like protein 8
NCBI Official Synonym Full Names
angiopoietin like 8
NCBI Official Symbol
ANGPTL8
NCBI Official Synonym Symbols
RIFL; TD26; PRO1185; PVPA599; C19orf80
NCBI Protein Information
angiopoietin-like protein 8
UniProt Protein Name
Angiopoietin-like protein 8
Protein Family
UniProt Gene Name
C19orf80
UniProt Entry Name
BETAT_HUMAN
Uniprot Description
EG624219: Hormone that specifically promotes pancreatic beta cell proliferation and beta cell mass expansion, thereby improving glucose tolerance. Promotes pancreatic beta cell proliferation without insulin resistance. Also acts as a blood lipid regulator by regulating serum triglyceride levels; possibly by promoting ANGPTL3 cleavage. In response to food intake. Interacts with ANGPTL3
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: extracellular region
Molecular Function: protein binding; hormone activity
Biological Process: fat cell differentiation; regulation of lipid metabolic process; regulation of lipoprotein metabolic process; cell maturation; cellular lipid metabolic process
Research Articles on ANGPTL8
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Product Notes
The Human ANGPTL8 c19orf80 (Catalog #AAA2708435) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2708435 ELISA Kit recognizes Human ANGPTL8. AAA Biotech's Angiopoietin Like Protein 8 (ANGPTL8) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the ANGPTL8 c19orf80 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angiopoietin Like Protein 8 (ANGPTL8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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