Typical Testing Data/Standard Curve (for reference only)
Human Cerebral Dopamine Neurotrophic Factor (CDNF) ELISA Kit | CDNF elisa kit
Human Cerebral Dopamine Neurotrophic Factor (CDNF) Mini Samples ELISA Kit
Reactivity
Human
Applications
ELISA
Synonyms
Cerebral Dopamine Neurotrophic Factor (CDNF); Human Cerebral Dopamine Neurotrophic Factor (CDNF) Mini Samples ELISA Kit; Cerebral Dopamine Neurotrophic Factor; ARMETL1; ARMET-like protein 1; Conserved Dopamine Neurotrophic Factor; Arginine-Rich; Mutated In Early Stage Tumors-Like 1; CDNF elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Cerebral Dopamine Neurotrophic Factor (CDNF).
No significant cross-reactivity or interference between Mini Samples Cerebral Dopamine Neurotrophic Factor (CDNF) and analogues was observed.
No significant cross-reactivity or interference between Mini Samples Cerebral Dopamine Neurotrophic Factor (CDNF) and analogues was observed.
Sequence Length
187
Applicable Applications for CDNF elisa kit
ELISA (EIA)
Assay Type
Double-antibody Sandwich
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.6-1,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 6.7pg/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Cerebral Dopamine Neurotrophic Factor (CDNF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Cerebral Dopamine Neurotrophic Factor (CDNF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Cerebral Dopamine Neurotrophic Factor (CDNF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Cerebral Dopamine Neurotrophic Factor (CDNF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Cerebral Dopamine Neurotrophic Factor (CDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Cerebral Dopamine Neurotrophic Factor (CDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Cerebral Dopamine Neurotrophic Factor (CDNF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
Cerebral dopamine neurotrophic factor
UniProt Protein Name
Cerebral dopamine neurotrophic factor
Protein Family
UniProt Gene Name
CDNF
UniProt Synonym Gene Names
ARMETL1
UniProt Entry Name
CDNF_HUMAN
Uniprot Description
ARMETL1: Trophic factor for dopamine neurons. Prevents the 6- hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra. Belongs to the ARMET family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 10p13
Cellular Component: extracellular region
Molecular Function: growth factor activity
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Product Notes
The Human CDNF cdnf (Catalog #AAA2708418) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2708418 ELISA Kit recognizes Human CDNF. AAA Biotech's Cerebral Dopamine Neurotrophic Factor (CDNF) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the CDNF cdnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cerebral Dopamine Neurotrophic Factor (CDNF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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