Mouse Platelet Derived Growth Factor C (PDGFC) ELISA Kit | PDGFC elisa kit

Mouse Platelet Derived Growth Factor C (PDGFC) Mini Samples ELISA Kit

Cat. Num. AAA2708406

• Gene Names: Pdgfc; SCDGF; PDGF-C; VEGF-E; AI647969; 1110064L01Rik
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Platelet Derived Growth Factor C (PDGFC); Mouse Platelet Derived Growth Factor C (PDGFC) Mini Samples ELISA Kit; Platelet Derived Growth Factor C; PDGF-C; SCDGF; VEGF-E; Fallotein; Spinal cord-derived growth factor; Platelet-derived growth factor C; latent form; receptor-binding form; PDGFC elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Platelet Derived Growth Factor C (PDGFC).; No significant cross-reactivity or interference between Mini Samples Platelet Derived Growth Factor C (PDGFC) and analogues was observed.
• Sequence Length: 345

• Applicable Applications for PDGFC elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Platelet Derived Growth Factor C (PDGFC) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Platelet Derived Growth Factor C (PDGFC) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Platelet Derived Growth Factor C (PDGFC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Platelet Derived Growth Factor C (PDGFC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Platelet Derived Growth Factor C (PDGFC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Platelet Derived Growth Factor C (PDGFC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 10242385
• NCBI GeneID: 54635
• NCBI Accession #: NP_064355.1
• NCBI GenBank Nucleotide #: NP_064355.1
• UniProt Accession #: Q8CI19
• NCBI Official Full Name: platelet-derived growth factor C isoform 1
• NCBI Official Synonym Full Names: platelet-derived growth factor, C polypeptide
• NCBI Official Symbol: Pdgfc
• NCBI Official Synonym Symbols: SCDGF; PDGF-C; VEGF-E; AI647969; 1110064L01Rik
• NCBI Protein Information: platelet-derived growth factor C
• UniProt Protein Name: Platelet-derived growth factor C
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: Pdgfc
• UniProt Synonym Gene Names: Scdgf; PDGF-C; SCDGF; PDGFC latent form; PDGFC receptor-binding form

Uniprot Description

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function.