Mouse Nuclear Factor Kappa B (NFkB) ELISA Kit | NFkB elisa kit

Mouse Nuclear Factor Kappa B (NFkB) Mini Samples ELISA Kit

Cat. Num. AAA2708370

• Gene Names: Nfkb1; p50; p105; NF-KB1; p50/p105; NF-kappaB; NF-kappaB1
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Nuclear Factor Kappa B (NFkB); Mouse Nuclear Factor Kappa B (NFkB) Mini Samples ELISA Kit; Nuclear Factor Kappa B; NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Nuclear Factor Kappa B (NFkB).; No significant cross-reactivity or interference between Mini Samples Nuclear Factor Kappa B (NFkB) and analogues was observed.
• Sequence Length: 971

• Applicable Applications for NFkB elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.059ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Nuclear Factor Kappa B (NFkB) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Nuclear Factor Kappa B (NFkB) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Nuclear Factor Kappa B (NFkB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Nuclear Factor Kappa B (NFkB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Nuclear Factor Kappa B (NFkB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Nuclear Factor Kappa B (NFkB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 341941170
• NCBI GeneID: 18033
• UniProt Accession #: P25799
• NCBI Official Full Name: Nuclear factor NF-kappa-B p105 subunit
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105
• NCBI Official Symbol: Nfkb1
• NCBI Official Synonym Symbols: p50; p105; NF-KB1; p50/p105; NF-kappaB; NF-kappaB1
• NCBI Protein Information: nuclear factor NF-kappa-B p105 subunit
• UniProt Protein Name: Nuclear factor NF-kappa-B p105 subunit
• UniProt Gene Name: Nfkb1
• UniProt Entry Name: NFKB1_MOUSE

Uniprot Description

NFkB-p105: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Cellular Component: nucleoplasm; protein complex; mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; heat shock protein binding; double-stranded DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; apoptosis; positive regulation of transcription, DNA-dependent; cellular response to stress; negative regulation of interleukin-12 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; lymph node development; regulation of transcription, DNA-dependent; response to cytokine stimulus; negative regulation of inflammatory response; negative regulation of cytokine production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis