Mouse Macrophage Inflammatory Protein 3 Beta (MIP3b) ELISA Kit | MIP3b elisa kit

Mouse Macrophage Inflammatory Protein 3 Beta (MIP3b) Mini Samples ELISA Kit

Cat. Num. AAA2708252

• Gene Names: Ccl19; ELC; CKb11; MIP3B; Scya19; exodus-3
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Macrophage Inflammatory Protein 3 Beta (MIP3b); Mouse Macrophage Inflammatory Protein 3 Beta (MIP3b) Mini Samples ELISA Kit; Macrophage Inflammatory Protein 3 Beta; CCL19; CKb11; ELC; MIP3-B; SCYA19; Chemokine C-C-Motif Ligand 19; Beta Chemokine Exodus-3; CK Beta-11; EBI1-Ligand Chemokine; Small Inducible Cytokine Subfamily A19; MIP3b elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Macrophage Inflammatory Protein 3 Beta (MIP3b).; No significant cross-reactivity or interference between Mini Samples Macrophage Inflammatory Protein 3 Beta (MIP3b) and analogues was observed.
• Sequence Length: 108

• Applicable Applications for MIP3b elisa kit: ELISA (EIA)
• Assay Type: Competitive Inhibition
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Macrophage Inflammatory Protein 3 Beta (MIP3b) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Macrophage Inflammatory Protein 3 Beta (MIP3b) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Macrophage Inflammatory Protein 3 Beta (MIP3b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Macrophage Inflammatory Protein 3 Beta (MIP3b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Macrophage Inflammatory Protein 3 Beta (MIP3b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Macrophage Inflammatory Protein 3 Beta (MIP3b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 6755422
• NCBI GeneID: 24047
• NCBI Accession #: NP_036018.1
• NCBI GenBank Nucleotide #: NP_036018.1
• UniProt Accession #: O70460
• NCBI Official Full Name: C-C motif chemokine 19
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 19
• NCBI Official Symbol: Ccl19
• NCBI Official Synonym Symbols: ELC; CKb11; MIP3B; Scya19; exodus-3
• NCBI Protein Information: C-C motif chemokine 19
• UniProt Protein Name: C-C motif chemokine 19
• UniProt Gene Name: Ccl19
• UniProt Synonym Gene Names: Elc; Scya19; EBI1 ligand chemokine; ELC
• UniProt Entry Name: CCL19_MOUSE

Uniprot Description

CCL19: May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: cell; intracellular; cytosol; external side of plasma membrane

Molecular Function: CCR chemokine receptor binding; chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding

Biological Process: positive regulation of protein kinase B signaling cascade; myeloid dendritic cell chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; release of sequestered calcium ion into cytosol; positive regulation of protein kinase activity; positive regulation of JNK cascade; dendritic cell chemotaxis; positive regulation of phosphoinositide 3-kinase activity; establishment of T cell polarity; positive regulation of NF-kappaB import into nucleus