Human Luteinizing Hormone (LH) ELISA Kit | LH elisa kit

Human Luteinizing Hormone (LH) Instant ELISA Kit

Cat. Num. AAA2708162

• Gene Names: UCP1; UCP; SLC25A7
• Reactivity: Human
• Applications: ELISA
• Synonyms: Luteinizing Hormone (LH); Human Luteinizing Hormone (LH) Instant ELISA Kit; Luteinizing Hormone; ICSH; Lutropin; Interstitial Cell Stimulating Hormone; LH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Instant Luteinizing Hormone (LH).; No significant cross-reactivity or interference between Instant Luteinizing Hormone (LH) and analogues was observed.
• Sequence Length: 307

• Applicable Applications for LH elisa kit: ELISA (EIA)
• Assay Type: Competitive Inhibition
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Luteinizing Hormone (LH) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Luteinizing Hormone (LH) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Luteinizing Hormone (LH). Standards or samples and HRP-labeled detection antibody specific to Instant Luteinizing Hormone (LH) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Luteinizing Hormone (LH), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Instant Luteinizing Hormone (LH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 11225256
• NCBI GeneID: 7350
• NCBI Accession #: NP_068605
• NCBI GenBank Nucleotide #: NP_068605.1
• UniProt Accession #: P25874
• NCBI Official Full Name: mitochondrial brown fat uncoupling protein 1
• NCBI Official Synonym Full Names: uncoupling protein 1
• NCBI Official Symbol: UCP1
• NCBI Official Synonym Symbols: UCP; SLC25A7
• NCBI Protein Information: mitochondrial brown fat uncoupling protein 1
• UniProt Protein Name: Mitochondrial brown fat uncoupling protein 1
• UniProt Gene Name: UCP1
• UniProt Synonym Gene Names: SLC25A7; UCP; UCP 1
• UniProt Entry Name: UCP1_HUMAN

NCBI Description

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq, Jul 2008]

Uniprot Description

UCP1: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial

Chromosomal Location of Human Ortholog: 4q28-q31

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: oxidative phosphorylation uncoupler activity

Biological Process: regulation of transcription from RNA polymerase II promoter; cellular metabolic process; proton transport; brown fat cell differentiation; mitochondrial transport

Disease: Obesity