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SDS-Page (Figure. SDS-PAGE)

Luteinizing Hormone Recombinant Protein | LH recombinant protein

Recombinant Luteinizing Hormone (LH)

Gene Names
UCP1; UCP; SLC25A7
Applications
SDS-Page, Western Blot
Purity
>80%
Synonyms
Luteinizing Hormone; Recombinant Luteinizing Hormone (LH); ICSH; Lutropin; Interstitial Cell Stimulating Hormone; LH recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
>80%
Form/Format
Freeze-dried powder
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Sequence Positions
Ala25~Ser116+GGGGS*3+Ser21~Leu141
Sequence Length
307
Applicable Applications for LH recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Source
Prokaryotic expression
Species
Human
Tag
N-terminal His Tag
Subcellular Location
Secreted
Isoelectric Point
8
Reconstitution
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

(Figure. SDS-PAGE)

SDS-Page (Figure. SDS-PAGE)

Gene Sequencing

(Figure. Gene Sequencing (Extract))

Gene Sequencing (Figure. Gene Sequencing (Extract))
Product Categories/Family for LH recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 54.3kDa
Accurate Molecular Mass: 54kDa
NCBI Official Full Name
mitochondrial brown fat uncoupling protein 1
NCBI Official Synonym Full Names
uncoupling protein 1
NCBI Official Symbol
UCP1
NCBI Official Synonym Symbols
UCP; SLC25A7
NCBI Protein Information
mitochondrial brown fat uncoupling protein 1
UniProt Protein Name
Mitochondrial brown fat uncoupling protein 1
UniProt Gene Name
UCP1
UniProt Synonym Gene Names
SLC25A7; UCP; UCP 1
UniProt Entry Name
UCP1_HUMAN

NCBI Description

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq, Jul 2008]

Uniprot Description

UCP1: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial

Chromosomal Location of Human Ortholog: 4q28-q31

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane

Molecular Function: oxidative phosphorylation uncoupler activity

Biological Process: regulation of transcription from RNA polymerase II promoter; cellular metabolic process; proton transport; brown fat cell differentiation; mitochondrial transport

Disease: Obesity

Research Articles on LH

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Product Notes

The LH ucp1 (Catalog #AAA2122633) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Ala25~Ser116+GGGGS*3+Ser21~Leu141. AAA Biotech's Luteinizing Hormone can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the LH ucp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Luteinizing Hormone, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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