Human Activation Induced Cytidine Deaminase (AICDA) ELISA Kit | AICDA elisa kit

Human Activation Induced Cytidine Deaminase (AICDA) ELISA Kit

Cat. Num. AAA2707739

• Gene Names: AICDA; AID; ARP2; CDA2; HIGM2; HEL-S-284
• Reactivity: Human
• Synonyms: Activation Induced Cytidine Deaminase (AICDA); Human Activation Induced Cytidine Deaminase (AICDA) ELISA Kit; ARP2; AID; CDA2; HIGM2; Cytidine aminohydrolase; Single-stranded DNA cytosine deaminase; AICDA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Activation Induced Cytidine Deaminase (AICDA).; No significant cross-reactivity or interference between Activation Induced Cytidine Deaminase (AICDA) and analogues was observed.
• Sequence Length: 198

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.112ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activation Induced Cytidine Deaminase (AICDA) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activation Induced Cytidine Deaminase (AICDA) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AICDA elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activation Induced Cytidine Deaminase (AICDA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activation Induced Cytidine Deaminase (AICDA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activation Induced Cytidine Deaminase (AICDA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activation Induced Cytidine Deaminase (AICDA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 23813666
• NCBI GeneID: 57379
• UniProt Accession #: Q9GZX7
• NCBI Official Full Name: Single-stranded DNA cytosine deaminase
• NCBI Official Synonym Full Names: activation induced cytidine deaminase
• NCBI Official Symbol: AICDA
• NCBI Official Synonym Symbols: AID; ARP2; CDA2; HIGM2; HEL-S-284
• NCBI Protein Information: single-stranded DNA cytosine deaminase
• UniProt Protein Name: Single-stranded DNA cytosine deaminase
• Protein Family: Single-stranded DNA cytosine deaminase
• UniProt Gene Name: AICDA
• UniProt Synonym Gene Names: AID
• UniProt Entry Name: AICDA_HUMAN

NCBI Description

This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq, Feb 2009]

Uniprot Description

AID: Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class- switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Strongly expressed in lymph nodes and tonsils. Belongs to the cytidine and deoxycytidylate deaminase family.

Protein type: EC 3.5.4.38; Hydrolase

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytoplasm; exosome (RNase complex); nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin protein ligase binding; cytidine deaminase activity

Biological Process: somatic diversification of immunoglobulins; B cell differentiation; somatic hypermutation of immunoglobulin genes; isotype switching; cytidine deamination; mRNA processing

Disease: Immunodeficiency With Hyper-igm, Type 2