Human Coproporphyrinogen Oxidase (CPOX) ELISA Kit | CPOX elisa kit

Human Coproporphyrinogen Oxidase (CPOX) ELISA Kit

Cat. Num. AAA2707699

• Gene Names: CPOX; COX; CPO; CPX; HCP
• Reactivity: Human
• Synonyms: Coproporphyrinogen Oxidase (CPOX); Human Coproporphyrinogen Oxidase (CPOX) ELISA Kit; HCP; CPO; CPX; Coproporphyrinogenase; Coproporphyria; Harderoporphyria; Oxygen-dependent coproporphyrinogen-III oxidase; mitochondrial; CPOX elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Coproporphyrinogen Oxidase (CPOX).; No significant cross-reactivity or interference between Coproporphyrinogen Oxidase (CPOX) and analogues was observed.
• Sequence Length: 454

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.055ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coproporphyrinogen Oxidase (CPOX) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coproporphyrinogen Oxidase (CPOX) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CPOX elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coproporphyrinogen Oxidase (CPOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coproporphyrinogen Oxidase (CPOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coproporphyrinogen Oxidase (CPOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coproporphyrinogen Oxidase (CPOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 67476671
• NCBI GeneID: 1371
• UniProt Accession #: P36551
• NCBI Official Full Name: Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
• NCBI Official Synonym Full Names: coproporphyrinogen oxidase
• NCBI Official Symbol: CPOX
• NCBI Official Synonym Symbols: COX; CPO; CPX; HCP
• NCBI Protein Information: oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
• UniProt Protein Name: Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
• Protein Family: Oxygen-dependent coproporphyrinogen-III oxidase
• UniProt Gene Name: CPOX
• UniProt Synonym Gene Names: CPO; CPX; COX; Coprogen oxidase; Coproporphyrinogenase
• UniProt Entry Name: HEM6_HUMAN

NCBI Description

The protein encoded by this gene is the sixth enzyme of the heme biosynthetic pathway. The encoded enzyme is soluble and found in the intermembrane space of mitochondria. This enzyme catalyzes the stepwise oxidative decarboxylation of coproporphyrinogen III to protoporphyrinogen IX, a precursor of heme. Defects in this gene are a cause of hereditary coproporphyria (HCP).[provided by RefSeq, Oct 2009]

Uniprot Description

CPOX: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III. Defects in CPOX are the cause of hereditary coproporphyria (HCP). HCP is an acute hepatic porphyria and an autosomal dominant disease characterized by neuropsychiatric disturbances and skin photosensitivity. Biochemically, there is an overexcretion of coproporphyrin III in the urine and in the feces. HCP is clinically characterized by attacks of abdominal pain, neurological disturbances, and psychiatric symptoms. The symptoms are generally manifested with rapid onset, and can be precipitated by drugs, alcohol, caloric deprivation, infection, endocrine factors or stress. A severe variant form is harderoporphyria, which is characterized by earlier onset attacks, massive excretion of harderoporphyrin in the feces, and a marked decrease of coproporphyrinogen IX oxidase activity. Belongs to the aerobic coproporphyrinogen-III oxidase family.

Protein type: Oxidoreductase; EC 1.3.3.3; Mitochondrial; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Chromosomal Location of Human Ortholog: 3q12

Cellular Component: mitochondrion; cytoplasm; mitochondrial inner membrane; mitochondrial intermembrane space

Molecular Function: coproporphyrinogen oxidase activity; protein homodimerization activity; structural constituent of eye lens

Biological Process: response to arsenic; response to methylmercury; response to lead ion; porphyrin metabolic process; response to insecticide; protoporphyrinogen IX biosynthetic process; response to iron ion; heme biosynthetic process

Disease: Coproporphyria, Hereditary