Human Taste Receptor Type 2 Member 5 (TAS2R5) ELISA Kit | TAS2R5 elisa kit

Human Taste Receptor Type 2 Member 5 (TAS2R5) ELISA Kit

Cat. Num. AAA2707661

• Gene Names: TAS2R5; T2R5
• Reactivity: Human
• Synonyms: Taste Receptor Type 2 Member 5 (TAS2R5); Human Taste Receptor Type 2 Member 5 (TAS2R5) ELISA Kit; T2R5; TAS2R5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Taste Receptor Type 2 Member 5 (TAS2R5).; No significant cross-reactivity or interference between Taste Receptor Type 2 Member 5 (TAS2R5) and analogues was observed.
• Sequence Length: 299

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.055ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Taste Receptor Type 2 Member 5 (TAS2R5) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Taste Receptor Type 2 Member 5 (TAS2R5) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TAS2R5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Taste Receptor Type 2 Member 5 (TAS2R5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Taste Receptor Type 2 Member 5 (TAS2R5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Taste Receptor Type 2 Member 5 (TAS2R5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Taste Receptor Type 2 Member 5 (TAS2R5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 9507173
• NCBI GeneID: 54429
• NCBI GenBank Nucleotide #: NP_061853.1
• UniProt Accession #: Q9NYW4
• NCBI Official Full Name: taste receptor type 2 member 5
• NCBI Official Synonym Full Names: taste 2 receptor member 5
• NCBI Official Symbol: TAS2R5
• NCBI Official Synonym Symbols: T2R5
• NCBI Protein Information: taste receptor type 2 member 5
• UniProt Protein Name: Taste receptor type 2 member 5
• Protein Family: Taste receptor
• UniProt Gene Name: TAS2R5
• UniProt Synonym Gene Names: T2R5
• UniProt Entry Name: TA2R5_HUMAN

NCBI Description

This gene encodes a bitter taste receptor; bitter taste receptors are members of the G protein-coupled receptor superfamily and are specifically expressed by taste receptor cells of the tongue and palate epithelia. Each of these apparently intronless taste receptor genes encodes a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is clustered with another 3 candidate taste receptor genes on chromosome 7 and is genetically linked to loci that influence bitter perception. [provided by RefSeq, Jul 2008]

Uniprot Description

TAS2R5: Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5. Belongs to the G-protein coupled receptor T2R family.

Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, T2R family

Chromosomal Location of Human Ortholog: 7q31.3-q32

Cellular Component: plasma membrane; integral to membrane

Molecular Function: bitter taste receptor activity; taste receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; sensory perception of taste; chemosensory behavior; detection of chemical stimulus involved in sensory perception of bitter taste; signal transduction