Human Hypoxia Inducible Factor 3 Alpha (HIF3a) ELISA Kit | HIF3a elisa kit

Human Hypoxia Inducible Factor 3 Alpha (HIF3a) ELISA Kit

Cat. Num. AAA2707632

• Gene Names: HIF3A; IPAS; MOP7; PASD7; HIF-3A; bHLHe17; HIF3-alpha-1
• Reactivity: Human
• Synonyms: Hypoxia Inducible Factor 3 Alpha (HIF3a); Human Hypoxia Inducible Factor 3 Alpha (HIF3a) ELISA Kit; HIF-3A; HIF-3A4; IPAS; MOP7; PASD7; Basic-helix-loop-helix-PAS protein MOP7; Class E basic helix-loop-helix protein 17; Inhibitory PAS domain protein; HIF3a elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 3 Alpha (HIF3a).; No significant cross-reactivity or interference between Hypoxia Inducible Factor 3 Alpha (HIF3a) and analogues was observed.
• Sequence Length: 669

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.059ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hypoxia Inducible Factor 3 Alpha (HIF3a) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hypoxia Inducible Factor 3 Alpha (HIF3a) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HIF3a elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 3 Alpha (HIF3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 3 Alpha (HIF3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Inducible Factor 3 Alpha (HIF3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxia Inducible Factor 3 Alpha (HIF3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 90421311
• NCBI GeneID: 64344
• NCBI Accession #: NP_690008.2
• NCBI GenBank Nucleotide #: NP_690008.2
• UniProt Accession #: Q9Y2N7
• NCBI Official Full Name: hypoxia-inducible factor 3-alpha isoform c
• NCBI Official Synonym Full Names: hypoxia inducible factor 3 subunit alpha
• NCBI Official Symbol: HIF3A
• NCBI Official Synonym Symbols: IPAS; MOP7; PASD7; HIF-3A; bHLHe17; HIF3-alpha-1
• NCBI Protein Information: hypoxia-inducible factor 3-alpha
• UniProt Protein Name: Hypoxia-inducible factor 3-alpha
• Protein Family: Hypoxia-inducible factor
• UniProt Gene Name: HIF3A
• UniProt Synonym Gene Names: BHLHE17; MOP7; PASD7; HIF-3-alpha; HIF3-alpha; bHLHe17; IPAS
• UniProt Entry Name: HIF3A_HUMAN

NCBI Description

The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

HIF3A: Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Transcription factor

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein dimerization activity; signal transducer activity; DNA binding; transcription coactivator activity; transcription factor activity; transcription corepressor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; signal transduction