Cattle Fibroblast Growth Factor 1 (FGF1) ELISA Kit | FGF1 elisa kit

Cattle Fibroblast Growth Factor 1 (FGF1) ELISA Kit

Cat. Num. AAA2707374

• Gene Names: FGF1; ECGF; EDGF; aFGF; FGF-1; HBGF-1; EDGF II
• Reactivity: Cattle
• Synonyms: Fibroblast Growth Factor 1 (FGF1); Cattle Fibroblast Growth Factor 1 (FGF1) ELISA Kit; ECGF; AFGF; ECGFA; ECGFB; FGF-Alpha; FGFA; HBGF1; Acidic Fibroblast Growth Factor; heparin-binding growth factor 1; Endothelial Cell Growth Factor; Beta; FGF1 elisa kit

• Reactivity: Cattle
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 1, Acidic (FGF1).; No significant cross-reactivity or interference between Fibroblast Growth Factor 1, Acidic (FGF1) and analogues was observed.
• Sequence Length: 155

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 62.5-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 26.6pg/mL.
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 1, Acidic (FGF1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF1 elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 1, Acidic (FGF1) and unlabeled Fibroblast Growth Factor 1, Acidic (FGF1) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 1, Acidic (FGF1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 1, Acidic (FGF1) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 27806633
• NCBI GeneID: 281160
• NCBI Accession #: NP_776480.1
• NCBI GenBank Nucleotide #: NP_776480.1
• UniProt Accession #: P03968
• NCBI Official Full Name: fibroblast growth factor 1
• NCBI Official Synonym Full Names: fibroblast growth factor 1
• NCBI Official Symbol: FGF1
• NCBI Official Synonym Symbols: ECGF; EDGF; aFGF; FGF-1; HBGF-1; EDGF II
• NCBI Protein Information: fibroblast growth factor 1
• UniProt Protein Name: Fibroblast growth factor 1
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF1
• UniProt Synonym Gene Names: AFGF; FGFA; HBGF-1; FGF-1; EDGF II; aFGF; ECGF; HBGF-1
• UniProt Entry Name: FGF1_BOVIN

Uniprot Description

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis ().