Human Defensin Beta 131 (DEFb131) ELISA Kit | DEFb131 elisa kit

Human Defensin Beta 131 (DEFb131) ELISA Kit

Cat. Num. AAA2707269

• Gene Names: DEFB131A; DEFB-31; DEFB131
• Reactivity: Human
• Synonyms: Defensin Beta 131 (DEFb131); Human Defensin Beta 131 (DEFb131) ELISA Kit; DEFB-31; DEFb131 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Defensin Beta 131 (DEFb131).; No significant cross-reactivity or interference between Defensin Beta 131 (DEFb131) and analogues was observed.
• Sequence Length: 70

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 5.6pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Beta 131 (DEFb131) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Beta 131 (DEFb131) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DEFb131 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 131 (DEFb131). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 131 (DEFb131). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 131 (DEFb131), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 131 (DEFb131) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 182888373
• NCBI GeneID: 644414
• UniProt Accession #: P59861
• Molecular Weight: 8,199 Da
• NCBI Official Full Name: Defensin, beta 131
• NCBI Official Synonym Full Names: defensin beta 131A
• NCBI Official Symbol: DEFB131A
• NCBI Official Synonym Symbols: DEFB-31; DEFB131
• NCBI Protein Information: beta-defensin 131A
• UniProt Protein Name: Beta-defensin 131A
• Protein Family: Beta-defensin
• UniProt Gene Name: DEFB131A
• UniProt Synonym Gene Names: DEFB-31

NCBI Description

Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. The antimicrobial protein encoded by this gene is secreted and is a member of the beta defensin protein family. Beta defensin genes are found in several clusters throughout the genome, with this gene mapping to a cluster at 4p16. [provided by RefSeq, Nov 2014]

Uniprot Description

Has antibacterial activity (Probable). Upon stimulation with lipoteichoic acid, promotes cytokines and chemokines production and secretion (PubMed:26649771).