Typical Testing Data/Standard Curve
Mouse Defensin Beta 119 ELISA Kit | DEFb119 elisa kit
Mouse Defensin Beta 119 (DEFb119) ELISA Kit
Gene Names
DEFB119; DEFB20; DEFB-19; DEFB-20; DEFB120; ESC42-RELA; ESC42-RELB
Reactivity
Mouse
Synonyms
Defensin Beta 119; Mouse Defensin Beta 119 (DEFb119) ELISA Kit; DEFB120; DEFB-19; DEFB-20; DEFb119 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Defensin Beta 119 (DEFb119).
No significant cross-reactivity or interference between Defensin Beta 119 (DEFb119) and analogues was observed.
No significant cross-reactivity or interference between Defensin Beta 119 (DEFb119) and analogues was observed.
Sequence Length
84
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates and Other Biological Fluids
Detection Range
15.62-1000pg/mL
Sensitivity
< 6.5pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Defensin Beta 119 (DEFb119) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Defensin Beta 119 (DEFb119) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for DEFb119 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 119 (DEFb119). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 119 (DEFb119). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 119 (DEFb119), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 119 (DEFb119) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for DEFb119 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
4,723 Da
NCBI Official Full Name
defensin beta 119
NCBI Official Synonym Full Names
defensin beta 119
NCBI Official Symbol
DEFB119
NCBI Official Synonym Symbols
DEFB20; DEFB-19; DEFB-20; DEFB120; ESC42-RELA; ESC42-RELB
NCBI Protein Information
beta-defensin 119
UniProt Protein Name
Beta-defensin 119
Protein Family
UniProt Gene Name
DEFB119
UniProt Synonym Gene Names
DEFB120; DEFB19; DEFB20; DEFB-19; DEFB-20
UniProt Entry Name
DB119_HUMAN
NCBI Description
This gene encodes a member of the beta subfamily of defensins. Beta-defensins are antimicrobial peptides that protect tissues and organs from infection by a variety of microorganisms. This gene is found in a cluster with other beta-defensin genes on the long arm of chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
Uniprot Description
defensin, beta 19: Has antibacterial activity (Potential). Belongs to the beta-defensin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 20q11.21
Cellular Component: cell surface; extracellular region
Biological Process: defense response to bacterium; innate immune response
Research Articles on DEFb119
Similar Products
Product Notes
The Mouse DEFb119 defb119 (Catalog #AAA2031943) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2031943 ELISA Kit recognizes Mouse DEFb119. It is sometimes possible for the material contained within the vial of "Defensin Beta 119, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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