Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) ELISA Kit | LIMS1 elisa kit

Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) ELISA Kit

Cat. Num. AAA2707198

• Gene Names: LIMS1; PINCH; PINCH1; PINCH-1
• Reactivity: Human
• Synonyms: LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1); Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) ELISA Kit; PINCH; PINCH1; Particularly interesting new Cys-His protein 1; Renal carcinoma antigen NY-REN-48; LIMS1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1).; No significant cross-reactivity or interference between LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) and analogues was observed.
• Sequence Length: 387

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LIMS1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 301601611
• NCBI GeneID: 3987
• NCBI Accession #: NP_001180414.1
• NCBI GenBank Nucleotide #: NM_001193485.2
• UniProt Accession #: P48059; Q53TE0; Q9BS44; B2RAJ4; B7Z483; B7Z7R3; B7Z907
• Molecular Weight: 41,571 Da
• NCBI Official Full Name: LIM and senescent cell antigen-like-containing domain protein 1 isoform a
• NCBI Official Synonym Full Names: LIM zinc finger domain containing 1
• NCBI Official Symbol: LIMS1
• NCBI Official Synonym Symbols: PINCH; PINCH1; PINCH-1
• NCBI Protein Information: LIM and senescent cell antigen-like-containing domain protein 1
• UniProt Protein Name: LIM and senescent cell antigen-like-containing domain protein 1
• Protein Family: LIM and senescent cell antigen-like-containing domain protein
• UniProt Gene Name: LIMS1
• UniProt Synonym Gene Names: PINCH; PINCH1; PINCH-1

NCBI Description

The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation.