Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) ELISA Kit | LIMS1 elisa kit

Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) ELISA Kit

Cat. Num. AAA2025064

• Gene Names: LIMS1; PINCH; PINCH1; PINCH-1
• Reactivity: Human
• Synonyms: LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1); Human LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1) ELISA Kit; PINCH; PINCH1; Particularly interesting new Cys-His protein 1; Renal carcinoma antigen NY-REN-48; LIMS1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LIMS1. No significant cross-reactivity or interference between LIMS1 and analogues was observed.
• Sequence Length: 387

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LIMS1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LIMS1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LIMS1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LIMS1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to LIMS1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to LIMS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIMS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LIMS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LIMS1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 301601611
• NCBI GeneID: 3987
• NCBI Accession #: NP_001180414.1
• NCBI GenBank Nucleotide #: NM_001193485.2
• Molecular Weight: 41,571 Da
• NCBI Official Full Name: LIM and senescent cell antigen-like-containing domain protein 1 isoform a
• NCBI Official Synonym Full Names: LIM and senescent cell antigen-like domains 1
• NCBI Official Symbol: LIMS1
• NCBI Official Synonym Symbols: PINCH; PINCH1; PINCH-1
• NCBI Protein Information: LIM and senescent cell antigen-like-containing domain protein 1
• UniProt Protein Name: LIM and senescent cell antigen-like-containing domain protein 1
• Protein Family: LIM and senescent cell antigen-like-containing domain protein
• UniProt Gene Name: LIMS1
• UniProt Synonym Gene Names: PINCH; PINCH1; PINCH-1
• UniProt Entry Name: LIMS1_HUMAN

NCBI Description

The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

LIMS1: Effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q12.3

Cellular Component: focal adhesion; protein complex; perinuclear region of cytoplasm; plasma membrane; intercellular junction; cytosol

Molecular Function: protein binding; zinc ion binding; protein kinase binding

Biological Process: intercellular junction assembly and maintenance; chordate embryonic development; protein heterooligomerization; cell-matrix adhesion; establishment and/or maintenance of cell polarity; cell aging; regulation of epithelial cell proliferation; cell-cell adhesion; establishment of protein localization; positive regulation of focal adhesion formation; epithelial to mesenchymal transition; negative regulation of transcription, DNA-dependent; positive regulation of GTPase activity; negative regulation of apoptosis