Human Sorcin (SRI) ELISA Kit | SRI elisa kit

Human Sorcin (SRI) ELISA Kit

Cat. Num. AAA2706319

• Gene Names: SRI; SCN; V19; CP22; CP-22
• Reactivity: Human
• Synonyms: Sorcin (SRI); Human Sorcin (SRI) ELISA Kit; SCN; CP-22; V19; 22 kDa protein; SRI elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sorcin (SRI).; No significant cross-reactivity or interference between Sorcin (SRI) and analogues was observed.
• Sequence Length: 180

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.119ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sorcin (SRI) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sorcin (SRI) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SRI elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sorcin (SRI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sorcin (SRI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sorcin (SRI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sorcin (SRI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 459836
• NCBI GeneID: 6717
• Molecular Weight: 20,006 Da
• NCBI Official Full Name: sorcin
• NCBI Official Synonym Full Names: sorcin
• NCBI Official Symbol: SRI
• NCBI Official Synonym Symbols: SCN; V19; CP22; CP-22
• NCBI Protein Information: sorcin
• UniProt Protein Name: Sorcin
• UniProt Gene Name: SRI
• UniProt Synonym Gene Names: CP22

NCBI Description

This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

Calcium-binding protein that modulates excitation-contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels.