Human Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2) ELISA Kit | HAND2 elisa kit

Human Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2) ELISA Kit

Cat. Num. AAA2705819

• Gene Names: HAND2; Hed; dHand; DHAND2; Thing2; bHLHa26
• Reactivity: Human
• Synonyms: Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2); Human Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2) ELISA Kit; Thing2; dHand; Hed; bHLHa26; Class A basic helix-loop-helix protein 26; Deciduum; heart; autonomic nervous system and neural crest derivatives-expressed protein 2; HAND2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2).; No significant cross-reactivity or interference between Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2) and analogues was observed.
• Sequence Length: 217

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HAND2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heart And Neural Crest Derivatives Expressed Protein 2 (HAND2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 12545384
• NCBI GeneID: 9464
• NCBI Accession #: NP_068808.1
• NCBI GenBank Nucleotide #: NM_021973.2
• UniProt Accession #: P61296; O95300; O95301; P97833; Q494T1; B6ECG9
• Molecular Weight: 20,269 Da
• NCBI Official Full Name: heart- and neural crest derivatives-expressed protein 2
• NCBI Official Synonym Full Names: heart and neural crest derivatives expressed 2
• NCBI Official Symbol: HAND2
• NCBI Official Synonym Symbols: Hed; dHand; DHAND2; Thing2; bHLHa26
• NCBI Protein Information: heart- and neural crest derivatives-expressed protein 2
• UniProt Protein Name: Heart- and neural crest derivatives-expressed protein 2
• Protein Family: Heart- and neural crest derivatives-expressed protein
• UniProt Gene Name: HAND2
• UniProt Synonym Gene Names: BHLHA26; DHAND; bHLHa26; dHAND

NCBI Description

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development. [provided by RefSeq, Jul 2008]

Uniprot Description

HAND2: Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3'.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 4q34.1

Cellular Component: nuclear chromatin; protein complex; transcription factor complex

Molecular Function: activating transcription factor binding; AT DNA binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription coactivator activity; transcription factor binding; transcription regulatory region DNA binding

Biological Process: adult heart development; angiogenesis; apoptotic process involved in heart morphogenesis; cardiac neural crest cell migration involved in outflow tract morphogenesis; cardiac right ventricle formation; embryonic digit morphogenesis; heart development; heart looping; in utero embryonic development; mesenchymal cell proliferation; negative regulation of cardiac muscle cell apoptotic process; negative regulation of DNA binding; negative regulation of gene expression; negative regulation of osteoblast differentiation; negative regulation of transcription factor activity; noradrenergic neuron differentiation; odontogenesis of dentine-containing teeth; palate development; peripheral nervous system neuron development; positive regulation of cardiac muscle hypertrophy; positive regulation of ERK1 and ERK2 cascade; positive regulation of gene expression; positive regulation of p38MAPK cascade; positive regulation of semaphorin-plexin signaling pathway involved in outflow tract morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter involved in norepinephrine biosynthetic process; positive regulation of transcription regulatory region DNA binding; positive regulation of transcription, DNA-templated; regulation of secondary heart field cardioblast proliferation; regulation of tissue remodeling; suckling behavior; sympathetic nervous system development; thymus development; tongue development; transcription from RNA polymerase II promoter