Human ATP Binding Cassette Transporter C11 (ABCC11) ELISA Kit | ABCC11 elisa kit

Human ATP Binding Cassette Transporter C11 (ABCC11) ELISA Kit

Cat. Num. AAA2705186

• Gene Names: ABCC11; WW; EWWD; MRP8
• Reactivity: Human
• Synonyms: ATP Binding Cassette Transporter C11 (ABCC11); Human ATP Binding Cassette Transporter C11 (ABCC11) ELISA Kit; ABC-C11; MRP8; EWWD; Multidrug resistance-associated protein 8; ABCC11 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter C11 (ABCC11).; No significant cross-reactivity or interference between ATP Binding Cassette Transporter C11 (ABCC11) and analogues was observed.
• Sequence Length: 1,344

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.103ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter C11 (ABCC11) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter C11 (ABCC11) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCC11 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter C11 (ABCC11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter C11 (ABCC11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter C11 (ABCC11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP Binding Cassette Transporter C11 (ABCC11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19851882
• NCBI GeneID: 85320
• Molecular Weight: 150,093 Da
• NCBI Official Full Name: ATP-binding cassette protein C11 isoform A
• NCBI Official Synonym Full Names: ATP binding cassette subfamily C member 11
• NCBI Official Symbol: ABCC11
• NCBI Official Synonym Symbols: WW; EWWD; MRP8
• NCBI Protein Information: ATP-binding cassette sub-family C member 11
• UniProt Protein Name: ATP-binding cassette sub-family C member 11
• Protein Family: ABC transporter C family
• UniProt Gene Name: ABCC11
• UniProt Synonym Gene Names: MRP8

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This ABC full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. The product of this gene participates in physiological processes involving bile acids, conjugated steroids, and cyclic nucleotides. In addition, a SNP in this gene is responsible for determination of human earwax type. This gene and family member ABCC12 are determined to be derived by duplication and are both localized to chromosome 16q12.1. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Participates in physiological processes involving bile acids, conjugated steroids and cyclic nucleotides (PubMed:12764137, PubMed:15537867). Enhances the cellular extrusion of cAMP and cGMP (PubMed:12764137, PubMed:15537867). Stimulates the ATP-dependent uptake of a range of physiological and synthetic lipophilic anions, including the glutathione S-conjugates leukotriene C4 and dinitrophenyl S-glutathione, steroid sulfates such as dehydroepiandrosterone 3-sulfate (DHEAS) and estrone 3-sulfate, glucuronides such as estradiol 17-beta-D-glucuronide (E217betaG), the monoanionic bile acids glycocholate and taurocholate, and methotrexate (PubMed:15537867, PubMed:25896536). Probably functions to secrete earwax (PubMed:16444273, PubMed:19383836). Required for the secretion of components contributing to axillary odor formation (PubMed:19710689).