Mouse Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) ELISA Kit | MYH7 elisa kit

Mouse Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) ELISA Kit

Cat. Num. AAA2705102

• Gene Names: MYH7; CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
• Reactivity: Mouse
• Synonyms: Myosin Heavy Chain 7; Cardiac Muscle; Beta (MYH7); Mouse Myosin Heavy Chain 7; Beta (MYH7) ELISA Kit; CMD1S; CMH1; MPD1; MYHCB; Myopathy; Distal 1; Myosin heavy chain slow isoform; Myosin heavy chain; cardiac muscle beta isoform; MYH7 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7).; No significant cross-reactivity or interference between Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) and analogues was observed.
• Sequence Length: 1,935

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 31.25-2000pg/mL
• Sensitivity: < 12.4pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYH7 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 83304912
• NCBI GeneID: 4625
• UniProt Accession #: P12883; Q14836; Q14837; Q14904; Q16579; Q2M1Y6; Q92679; Q9H1D5; Q9UDA2; Q9UMM8; A2TDB6; B6D424
• Molecular Weight: 223,097 Da
• NCBI Official Full Name: Myosin-7
• NCBI Official Synonym Full Names: myosin heavy chain 7
• NCBI Official Symbol: MYH7
• NCBI Official Synonym Symbols: CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
• NCBI Protein Information: myosin-7
• UniProt Protein Name: Myosin-7
• Protein Family: Myosin
• UniProt Gene Name: MYH7
• UniProt Synonym Gene Names: MYHCB; MyHC-slow; MyHC-beta

NCBI Description

Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.