Slit Homolog 3 (Slit3) ELISA Kit | Slit3 elisa kit

Multi-species Slit Homolog 3 (Slit3) ELISA Kit

Cat. Num. AAA2705065

• Gene Names: SLIT3; MEGF5; SLIL2; SLIT1; slit2; Slit-3
• Reactivity: Human, Mouse, Rat
• Synonyms: Slit Homolog 3 (Slit3); Multi-species Slit Homolog 3 (Slit3) ELISA Kit; MEGF5; SLIL2; Multiple epidermal growth factor-like domains protein 5; Slit3 elisa kit

• Reactivity: Human, Mouse, Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Slit Homolog 3 (Slit3).; No significant cross-reactivity or interference between Slit Homolog 3 (Slit3) and analogues was observed.
• Sequence Length: 1,530

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Slit Homolog 3 (Slit3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Slit Homolog 3 (Slit3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Slit3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Slit Homolog 3 (Slit3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Slit Homolog 3 (Slit3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Slit Homolog 3 (Slit3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Slit Homolog 3 (Slit3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11321571
• NCBI GeneID: 6586
• NCBI Accession #: NP_003053.1
• NCBI GenBank Nucleotide #: NM_003062.3
• UniProt Accession #: O75094; O95804; Q9UFH5; A6H8U9; J3KNP3
• Molecular Weight: 168,562 Da
• NCBI Official Full Name: slit homolog 3 protein isoform 2
• NCBI Official Synonym Full Names: slit guidance ligand 3
• NCBI Official Symbol: SLIT3
• NCBI Official Synonym Symbols: MEGF5; SLIL2; SLIT1; slit2; Slit-3
• NCBI Protein Information: slit homolog 3 protein
• UniProt Protein Name: Slit homolog 3 protein
• Protein Family: Slit homolog 3 protein
• UniProt Gene Name: SLIT3
• UniProt Synonym Gene Names: KIAA0814; MEGF5; SLIL2; Slit-3; Multiple EGF-like domains protein 5

NCBI Description

The protein encoded by this gene is secreted, likely interacting with roundabout homolog receptors to effect cell migration. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.