Human Optineurin (OPTN) ELISA Kit | OPTN elisa kit

Human Optineurin (OPTN) ELISA Kit

Cat. Num. AAA2704623

• Gene Names: OPTN; NRP; FIP2; HIP7; HYPL; ALS12; GLC1E; TFIIIA-INTP
• Reactivity: Human
• Synonyms: Optineurin (OPTN); Human Optineurin (OPTN) ELISA Kit; NRP; FIP2; GLC1E; HIP7; HYPL; TFIIIA-INTP; Glaucoma 1; Open Angle; E(Adult-Onset); Huntingtin-interacting protein 7; Optic neuropathy-inducing protein; E3-14.7K-interacting protein; OPTN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Optineurin (OPTN).; No significant cross-reactivity or interference between Optineurin (OPTN) and analogues was observed.
• Sequence Length: 577

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Optineurin (OPTN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Optineurin (OPTN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OPTN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Optineurin (OPTN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Optineurin (OPTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Optineurin (OPTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Optineurin (OPTN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 56549111
• NCBI GeneID: 10133
• NCBI Accession #: NP_001008214.1
• NCBI GenBank Nucleotide #: NM_001008213.1
• UniProt Accession #: Q96CV9; Q5T672; Q5T673; Q5T674; Q5T675; Q7LDL9; Q8N562; Q9UET9; Q9UEV4; B3KP00; D3DRS4; D3DRS8
• Molecular Weight: 59,559 Da
• NCBI Official Full Name: optineurin
• NCBI Official Synonym Full Names: optineurin
• NCBI Official Symbol: OPTN
• NCBI Official Synonym Symbols: NRP; FIP2; HIP7; HYPL; ALS12; GLC1E; TFIIIA-INTP
• NCBI Protein Information: optineurin
• UniProt Protein Name: Optineurin
• Protein Family: Optineurin
• UniProt Gene Name: OPTN
• UniProt Synonym Gene Names: FIP2; GLC1E; HIP7; HYPL; NRP; HIP-7; TFIIIA-IntP

NCBI Description

This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

optineurin: Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). May constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E). Primary open angle glaucoma (POAG) is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). Defects in OPTN are the cause of amyotrophic lateral sclerosis type 12 (ALS12). It is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: autophagic vacuole; cytoplasm; cytoplasmic vesicle; cytosol; Golgi apparatus; Golgi membrane; nucleoplasm; nucleus; perinuclear region of cytoplasm; recycling endosome membrane; trans-Golgi network

Molecular Function: identical protein binding; metal ion binding; polyubiquitin modification-dependent protein binding; protein binding; protein binding, bridging; protein C-terminus binding; Rab GTPase binding

Biological Process: autophagy; cell death; cellular response to unfolded protein; defense response to Gram-negative bacterium; G2/M transition of mitotic cell cycle; Golgi organization and biogenesis; Golgi ribbon formation; Golgi to plasma membrane protein transport; innate immune response; negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of receptor recycling; parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization; positive regulation of autophagy; positive regulation of xenophagy; protein targeting to Golgi; regulation of I-kappaB kinase/NF-kappaB cascade; signal transduction

Disease: Amyotrophic Lateral Sclerosis 12; Glaucoma, Normal Tension, Susceptibility To; Glaucoma, Primary Open Angle