Human Bcl2 Antagonist/Killer 1 (BAK1) ELISA Kit | BAK1 elisa kit

Human Bcl2 Antagonist/Killer 1 (BAK1) ELISA Kit

Cat. Num. AAA2704369

• Gene Names: BAK1; BAK; CDN1; BCL2L7; BAK-LIKE
• Reactivity: Human
• Synonyms: Bcl2 Antagonist/Killer 1 (BAK1); Human Bcl2 Antagonist/Killer 1 (BAK1) ELISA Kit; BAK; BAK-LIKE; BCL2L7; CDN1; Bcl-2 Homologous Antagonist/Killer; Apoptosis regulator BAK; Bcl-2-like protein 7; BAK1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bcl2 Antagonist/Killer 1 (BAK1).; No significant cross-reactivity or interference between Bcl2 Antagonist/Killer 1 (BAK1) and analogues was observed.
• Sequence Length: 153

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.239ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2 Antagonist/Killer 1 (BAK1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2 Antagonist/Killer 1 (BAK1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BAK1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Antagonist/Killer 1 (BAK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Antagonist/Killer 1 (BAK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Antagonist/Killer 1 (BAK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Antagonist/Killer 1 (BAK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502363
• NCBI GeneID: 578
• NCBI Accession #: NP_001179.1
• NCBI GenBank Nucleotide #: NM_001188.3
• UniProt Accession #: Q16611; Q6I9T6; Q92533; C0H5Y7
• Molecular Weight: 16,872 Da
• NCBI Official Full Name: bcl-2 homologous antagonist/killer
• NCBI Official Synonym Full Names: BCL2 antagonist/killer 1
• NCBI Official Symbol: BAK1
• NCBI Official Synonym Symbols: BAK; CDN1; BCL2L7; BAK-LIKE
• NCBI Protein Information: bcl-2 homologous antagonist/killer
• UniProt Protein Name: Bcl-2 homologous antagonist/killer
• Protein Family: Bcl-2 homologous antagonist/killer
• UniProt Gene Name: BAK1
• UniProt Synonym Gene Names: BAK; BCL2L7; CDN1; Bcl2-L-7

NCBI Description

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008]

Uniprot Description

In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.