Mouse Macrophage Migration Inhibitory Factor (MIF) ELISA Kit | MIF elisa kit

Mouse Macrophage Migration Inhibitory Factor (MIF) ELISA Kit

Cat. Num. AAA2702301

• Gene Names: MIF; GIF; GLIF; MMIF
• Reactivity: Mouse
• Synonyms: Macrophage Migration Inhibitory Factor (MIF); Mouse Macrophage Migration Inhibitory Factor (MIF) ELISA Kit; GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase; MIF elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Macrophage Migration Inhibitory Factor (MIF).; No significant cross-reactivity or interference between Macrophage Migration Inhibitory Factor (MIF) and analogues was observed.
• Sequence Length: 115

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 31.2-2000pg/mL
• Sensitivity: < 12.3pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Migration Inhibitory Factor (MIF) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Migration Inhibitory Factor (MIF) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MIF elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Migration Inhibitory Factor (MIF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Migration Inhibitory Factor (MIF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Migration Inhibitory Factor (MIF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Migration Inhibitory Factor (MIF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505185
• NCBI GeneID: 4282
• NCBI Accession #: NP_002406.1
• NCBI GenBank Nucleotide #: NM_002415.1
• UniProt Accession #: P14174; Q2V4Y5; Q6FHV0; A5Z1R8; B2R4S3
• Molecular Weight: 12,476 Da
• NCBI Official Full Name: macrophage migration inhibitory factor
• NCBI Official Synonym Full Names: macrophage migration inhibitory factor
• NCBI Official Symbol: MIF
• NCBI Official Synonym Symbols: GIF; GLIF; MMIF
• NCBI Protein Information: macrophage migration inhibitory factor
• UniProt Protein Name: Macrophage migration inhibitory factor
• Protein Family: Macrophage migration inhibitory factor
• UniProt Gene Name: MIF
• UniProt Synonym Gene Names: GLIF; MMIF; MIF; GIF

NCBI Description

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]

Uniprot Description

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.