Human Bone Morphogenetic Protein Receptor 2 (BMPR2) ELISA Kit | BMPR2 elisa kit

Human Bone Morphogenetic Protein Receptor 2 (BMPR2) ELISA Kit

Cat. Num. AAA2700764

• Gene Names: BMPR2; BMR2; PPH1; BMPR3; BRK-3; POVD1; T-ALK; BMPR-II
• Reactivity: Human
• Synonyms: Bone Morphogenetic Protein Receptor 2 (BMPR2); Human Bone Morphogenetic Protein Receptor 2 (BMPR2) ELISA Kit; BMPRII; BMPR-II; BRK3; T-ALK; BMPR3; PPH1; Primary Pulmonary Hypertension 1; BMP type II receptor; BMPR2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein Receptor 2 (BMPR2).; No significant cross-reactivity or interference between Bone Morphogenetic Protein Receptor 2 (BMPR2) and analogues was observed.
• Sequence Length: 1038

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.124ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 2 (BMPR2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 2 (BMPR2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMPR2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein Receptor 2 (BMPR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein Receptor 2 (BMPR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein Receptor 2 (BMPR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein Receptor 2 (BMPR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15451916
• NCBI GeneID: 659
• NCBI Accession #: NP_001195.2
• NCBI GenBank Nucleotide #: NM_001204.6
• UniProt Accession #: Q13873; Q13161; Q16569; Q4ZG08; Q53SA5; Q585T8
• Molecular Weight: 59,963 Da
• NCBI Official Full Name: bone morphogenetic protein receptor type-2
• NCBI Official Synonym Full Names: bone morphogenetic protein receptor type 2
• NCBI Official Symbol: BMPR2
• NCBI Official Synonym Symbols: BMR2; PPH1; BMPR3; BRK-3; POVD1; T-ALK; BMPR-II
• NCBI Protein Information: bone morphogenetic protein receptor type-2
• UniProt Protein Name: Bone morphogenetic protein receptor type-2
• Protein Family: Bone morphogenetic protein receptor
• UniProt Gene Name: BMPR2
• UniProt Synonym Gene Names: PPH1; BMP type-2 receptor; BMPR-2; BMP type II receptor; BMPR-II

NCBI Description

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of two different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension, both familial and fenfluramine-associated, and with pulmonary venoocclusive disease. [provided by RefSeq, Jul 2008]

Uniprot Description

BMPR2: a serine/threonine-protein kinase receptor for bone morphogenetic protein (BMP). Binds to BMP-7, BMP-2 and, less efficiently, BMP-4. Binding is weak but enhanced by the presence of type I receptors for BMPs. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Defects in BMPR2 are the cause of primary pulmonary hypertension (PPH1), a weakly penetrant dominant disorder, associated with lesions in pulmonary arterioles, elevated pulmonary arterial pressure, and right ventricular failure.

Protein type: Cell cycle regulation; EC 2.7.11.30; Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; STKR family; TKL group; Type2 subfamily

Chromosomal Location of Human Ortholog: 2q33.1-q33.2

Cellular Component: apical plasma membrane; basal plasma membrane; caveola; cell soma; cell surface; cell-cell adherens junction; cytoplasm; dendrite; extracellular space; integral component of plasma membrane; nucleoplasm; plasma membrane; postsynaptic density; spanning component of plasma membrane

Molecular Function: activin receptor activity, type II; ATP binding; BMP receptor activity; growth factor binding; metal ion binding; protein binding; signal transducer, downstream of receptor, with serine/threonine kinase activity

Biological Process: activin receptor signaling pathway; alveolus development; anterior/posterior pattern specification; atrioventricular valve morphogenesis; blood vessel remodeling; BMP signaling pathway; brain development; cardiac muscle tissue development; cellular response to BMP stimulus; cellular response to starvation; chondrocyte development; endocardial cushion development; endothelial cell apoptotic process; endothelial cell proliferation; limb development; lymphangiogenesis; maternal placenta development; mesoderm formation; mitral valve morphogenesis; negative regulation of cell growth; negative regulation of cell proliferation involved in heart valve morphogenesis; negative regulation of chondrocyte proliferation; negative regulation of DNA biosynthetic process; negative regulation of systemic arterial blood pressure; negative regulation of vasoconstriction; outflow tract morphogenesis; outflow tract septum morphogenesis; positive regulation of axon extension involved in axon guidance; positive regulation of BMP signaling pathway; positive regulation of bone mineralization; positive regulation of endothelial cell migration; positive regulation of endothelial cell proliferation; positive regulation of epithelial cell migration; positive regulation of ossification; positive regulation of osteoblast differentiation; positive regulation of pathway-restricted SMAD protein phosphorylation; positive regulation of transcription from RNA polymerase II promoter; proteoglycan biosynthetic process; regulation of cell proliferation; regulation of lung blood pressure; semi-lunar valve development; transcription from RNA polymerase II promoter; transmembrane receptor protein serine/threonine kinase signaling pathway; tricuspid valve morphogenesis; vascular endothelial growth factor receptor signaling pathway

Disease: Pulmonary Hypertension, Primary, 1; Pulmonary Venoocclusive Disease 1, Autosomal Dominant