Typical Testing Data/Standard Curve (for reference only)
Sheep CX43 ELISA Kit | CX43 elisa kit
Sheep CX43 (Connexin 43) ELISA Kit
Gene Names
cx43; gja1; cx43a1; zfCx43.3
Reactivity
Sheep
Synonyms
CX43; Sheep CX43 (Connexin 43) ELISA Kit; CX43 elisa kit
Reactivity
Sheep
Specificity
This kit recognizes natural and recombinant Sheep CX43. No significant cross-reactivity or interference between Sheep CX43 and analogues was observed.
Sequence Length
282
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.781-50ng/mL
Sensitivity
Min: 0.469ng/mL; Max: 50ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CX43 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep CX43 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CX43. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CX43 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CX43, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CX43. You can calculate the concentration of CX43 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CX43. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CX43 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CX43, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CX43. You can calculate the concentration of CX43 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
39,127 Da
NCBI Official Full Name
Cx43 protein
NCBI Official Synonym Full Names
connexin 43
NCBI Official Symbol
cx43
NCBI Official Synonym Symbols
gja1; cx43a1; zfCx43.3
NCBI Protein Information
gap junction alpha-1 protein; connexin-43; etID309742.9; gap junction protein, alpha 1; shf; short fin protein; sof
UniProt Protein Name
Gap junction alpha-1 protein
UniProt Gene Name
gja1
UniProt Synonym Gene Names
cx43; shf; sof; Cx43
UniProt Entry Name
CXA1_DANRE
Uniprot Description
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Plays an essential role in gap junction communication in the ventricles ().
Research Articles on CX43
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Product Notes
The Sheep CX43 gja1 (Catalog #AAA2500809) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2500809 ELISA Kit recognizes Sheep CX43. It is sometimes possible for the material contained within the vial of "CX43, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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