Human Relaxin (RLN) ELISA Kit | RLN elisa kit

Human Relaxin (RLN) ELISA Kit

Cat. Num. AAA2700477

• Gene Names: RLN1; H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• Reactivity: Human
• Synonyms: Relaxin (RLN); Human Relaxin (RLN) ELISA Kit; RLXH1; Relaxin H1; Prorelaxin H1; RLN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Relaxin (RLN).; No significant cross-reactivity or interference between Relaxin (RLN) and analogues was observed.
• Sequence Length: 185

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 24.69-2,000pg/mL
• Sensitivity: < 8.92pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Relaxin (RLN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Relaxin (RLN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RLN elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Relaxin (RLN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Relaxin (RLN) and unlabeled Relaxin (RLN) (Standards or samples) with the pre-coated antibody specific to Relaxin (RLN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Relaxin (RLN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Relaxin (RLN) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 490056
• NCBI GeneID: 6013
• UniProt Accession #: P04808; Q99936; Q9UQJ1
• Molecular Weight: 13,011 Da
• NCBI Official Full Name: relaxin
• NCBI Official Synonym Full Names: relaxin 1
• NCBI Official Symbol: RLN1
• NCBI Official Synonym Symbols: H1; H1RLX; RLXH1; bA12D24.3.1; bA12D24.3.2
• NCBI Protein Information: prorelaxin H1
• UniProt Protein Name: Prorelaxin H1
• Protein Family: Prorelaxin
• UniProt Gene Name: RLN1

NCBI Description

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In humans there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3, where RLN1 and RLN2 share high sequence homology. The protein encoded by this gene is synthesized as a single-chain polypeptide but the active form consists of an A chain and a B chain linked by disulfide bonds. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. [provided by RefSeq, Jan 2013]

Uniprot Description

Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.