Goat Adrenocorticotropic Hormone (ACTH) ELISA Kit | ACTH elisa kit

Goat Adrenocorticotropic Hormone (ACTH) ELISA Kit

Cat. Num. AAA2700342

• Gene Names: Pomc; BE; Npp; ACTH; Clip; Pomc1; Pomc-1; Beta-LPH; alphaMSH; beta-MSH; Gamma-LPH; alpha-MSH; gamma-MSH
• Reactivity: Goat
• Synonyms: Adrenocorticotropic Hormone (ACTH); Goat Adrenocorticotropic Hormone (ACTH) ELISA Kit; Corticotropin; Adrenocorticotropin; ACTH elisa kit

• Reactivity: Goat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH).; No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed.
• Sequence Length: 39

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 12.35-1000pg/mL
• Sensitivity: < 4.92pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACTH elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adrenocorticotropic Hormone (ACTH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adrenocorticotropic Hormone (ACTH) and unlabeled Adrenocorticotropic Hormone (ACTH) (Standards or samples) with the pre-coated antibody specific to Adrenocorticotropic Hormone (ACTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 191659
• NCBI GeneID: 18976
• Molecular Weight: 26,707 Da
• NCBI Official Full Name: adrenocorticotropic hormone, partial
• NCBI Official Synonym Full Names: pro-opiomelanocortin-alpha
• NCBI Official Symbol: Pomc
• NCBI Official Synonym Symbols: BE; Npp; ACTH; Clip; Pomc1; Pomc-1; Beta-LPH; alphaMSH; beta-MSH; Gamma-LPH; alpha-MSH; gamma-MSH
• NCBI Protein Information: pro-opiomelanocortin
• UniProt Protein Name: Pro-opiomelanocortin
• UniProt Gene Name: Pomc
• UniProt Synonym Gene Names: Pomc1; POMC; ACTH; Alpha-MSH; CLIP; Beta-MSH

NCBI Description

This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing. Processing yields several biologically active peptides, which are involved in diverse cellular functions, such as energy homeostasis, steroidogenesis, and increased melanin production in melanocytes. In mouse deficiency of this gene is associated with obesity, defects in adrenal development, and altered pigmentation. A pseudogene of this gene is located on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

Corticotropin: Stimulates the adrenal glands to release cortisol.