Porcine Motilin (MTL) ELISA Kit | MTL elisa kit

Porcine Motilin (MTL) ELISA Kit

Cat. Num. AAA266927

• Reactivity: Porcine
• Synonyms: Motilin (MTL); Porcine Motilin (MTL) ELISA Kit; MTL elisa kit

• Reactivity: Porcine
• Sequence Length: 115

• Samples: Porcine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 1000 pg/mL-15.6 pg/mL
• Sensitivity: Up to 5 pg/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MTL elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Porcine MTL monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 386955
• NCBI GeneID: 4295
• Molecular Weight: 12,849 Da
• NCBI Official Full Name: motilin
• NCBI Official Synonym Full Names: motilin
• NCBI Official Symbol: MLN
• NCBI Protein Information: promotilin; prepromotilin
• UniProt Protein Name: Promotilin
• UniProt Gene Name: MLN
• UniProt Synonym Gene Names: MAP
• UniProt Entry Name: MOTI_HUMAN

NCBI Description

This gene encodes a small peptide hormone that is secreted by cells of the small intestine to regulate gastrointestinal contractions and motility. Proteolytic processing of the secreted protein produces the mature peptide and a byproduct referred to as motilin-associated peptide (MAP). Three transcript variants encoding different preproprotein isoforms but the same mature peptide have been found for this gene. [provided by RefSeq, May 2010]

Uniprot Description

MLN: Plays an important role in the regulation of interdigestive gastrointestinal motility and indirectly causes rhythmic contraction of duodenal and colonic smooth muscle. Belongs to the motilin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular region

Molecular Function: hormone activity; receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling