Rat growth-associated protein 43 (GAP-43) ELISA Kit | GAP-43 elisa kit

Rat growth-associated protein 43 (GAP-43) ELISA Kit

Cat. Num. AAA266374

• Gene Names: GAP43; B-50; PP46
• Reactivity: Rat
• Synonyms: growth-associated protein 43 (GAP-43); Rat growth-associated protein 43 (GAP-43) ELISA Kit; GAP-43 elisa kit

• Reactivity: Rat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 238

• Samples: Serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/ml-0.156 ng/ml
• Sensitivity: Up to 0.05 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Related Product Information for GAP-43 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat GAP-43 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 119600000
• NCBI GeneID: 2596
• Molecular Weight: 28,766 Da
• NCBI Official Full Name: growth associated protein 43
• NCBI Official Synonym Full Names: growth associated protein 43
• NCBI Official Symbol: GAP43
• NCBI Official Synonym Symbols: B-50; PP46
• NCBI Protein Information: neuromodulin; protein F1; neural phosphoprotein B-50; growth-associated protein 43; axonal membrane protein GAP-43; calmodulin-binding protein P-57; nerve growth-related peptide GAP43; neuron growth-associated protein 43
• UniProt Protein Name: Neuromodulin
• UniProt Gene Name: GAP43
• UniProt Entry Name: NEUM_HUMAN

NCBI Description

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GAP43: This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Belongs to the neuromodulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: filopodium membrane; axon; cytoplasm; postsynaptic density; plasma membrane; cell junction

Molecular Function: calmodulin binding; protein binding

Biological Process: cell fate commitment; glial cell differentiation; tissue regeneration; regulation of growth; axon choice point recognition; protein kinase C activation; response to wounding; regulation of filopodium formation