Mouse Prostaglandin E2 (PGE2) ELISA Kit | PGE2 elisa kit

Mouse Prostaglandin E2 (PGE2) ELISA Kit

Cat. Num. AAA266212

• Gene Names: PTGER4; EP4; EP4R
• Reactivity: Mouse
• Synonyms: Prostaglandin E2 (PGE2); Mouse Prostaglandin E2 (PGE2) ELISA Kit; PGE2 elisa kit

• Reactivity: Mouse
• Sequence Length: 488

• Samples: Mouse serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 500 pg/mL-7.8 pg/mL
• Sensitivity: Up to 1 pg/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for PGE2 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse PGE2 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse PGE2 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4506259
• NCBI GeneID: 5734
• NCBI Accession #: NP_000949.1
• NCBI GenBank Nucleotide #: NM_000958.2
• Molecular Weight: 53,119 Da
• NCBI Official Full Name: prostaglandin E2 receptor EP4 subtype
• NCBI Official Synonym Full Names: prostaglandin E receptor 4 (subtype EP4)
• NCBI Official Symbol: PTGER4
• NCBI Official Synonym Symbols: EP4; EP4R
• NCBI Protein Information: prostaglandin E2 receptor EP4 subtype; prostanoid EP4 receptor; PGE receptor EP4 subtype; PGE receptor, EP4 subtype; PGE2 receptor EP4 subtype
• UniProt Protein Name: Prostaglandin E2 receptor EP4 subtype
• UniProt Gene Name: PTGER4
• UniProt Synonym Gene Names: PTGER2; PGE receptor EP4 subtype; PGE2 receptor EP4 subtype
• UniProt Entry Name: PE2R4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor can activate T-cell factor signaling. It has been shown to mediate PGE2 induced expression of early growth response 1 (EGR1), regulate the level and stability of cyclooxygenase-2 mRNA, and lead to the phosphorylation of glycogen synthase kinase-3. Knockout studies in mice suggest that this receptor may be involved in the neonatal adaptation of circulatory system, osteoporosis, as well as initiation of skin immune responses. [provided by RefSeq, Jul 2008]

Uniprot Description

PTGER4: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: plasma membrane; integral to membrane

Molecular Function: protein binding; prostaglandin E receptor activity

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; T-helper cell differentiation; response to mechanical stimulus; regulation of stress fiber formation; negative regulation of inflammatory response; negative regulation of cytokine secretion; JNK cascade; response to lipopolysaccharide; immune response; negative regulation of integrin activation; regulation of ossification; positive regulation of inflammatory response; positive regulation of cytokine secretion