Rat Glycogen phosphorylase BB (GP-BB) ELISA Kit | GP-BB elisa kit

Rat Glycogen phosphorylase BB (GP-BB) ELISA Kit

Cat. Num. AAA264178

• Gene Names: PYGB; GPBB
• Reactivity: Rat
• Synonyms: Glycogen phosphorylase BB (GP-BB); Rat Glycogen phosphorylase BB (GP-BB) ELISA Kit; GP-BB elisa kit

• Reactivity: Rat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 843

• Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant GP-BB concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GP-BB elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat GP-BB monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 21361370
• NCBI GeneID: 5834
• NCBI Accession #: NP_002853.2
• NCBI GenBank Nucleotide #: NM_002862.3
• Molecular Weight: 96,696 Da
• NCBI Official Full Name: glycogen phosphorylase, brain form
• NCBI Official Synonym Full Names: phosphorylase, glycogen; brain
• NCBI Official Symbol: PYGB
• NCBI Official Synonym Symbols: GPBB
• NCBI Protein Information: glycogen phosphorylase, brain form; glycogen phosphorylase B
• UniProt Protein Name: Glycogen phosphorylase, brain form
• UniProt Gene Name: PYGB
• UniProt Entry Name: PYGB_HUMAN

NCBI Description

The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008]

Uniprot Description

PYGB: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Belongs to the glycogen phosphorylase family.

Protein type: Transferase; Kinase, other; Carbohydrate Metabolism - starch and sucrose; EC 2.4.1.1; Phosphorylase

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: membrane; cytoplasm

Molecular Function: glycogen phosphorylase activity; pyridoxal phosphate binding

Biological Process: glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis