Porcine Gastrin Releasing Peptide (GRP) ELISA Kit | GRP elisa kit

Porcine Gastrin Releasing Peptide (GRP) ELISA Kit

Cat. Num. AAA263400

• Gene Names: GRP; BN; GRP-10; proGRP; preproGRP
• Reactivity: Porcine
• Synonyms: Gastrin Releasing Peptide (GRP); Porcine Gastrin Releasing Peptide (GRP) ELISA Kit; GRP elisa kit

• Reactivity: Porcine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 138

• Samples: Porcine Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Gm-Csf Concentration
• Assay Type: Sandwich
• Detection Range: 1000 pg/ml-15.6 pg/ml
• Sensitivity: 5 pg/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRP elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine GM-CSF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 60498999
• NCBI GeneID: 2922
• NCBI Accession #: NP_001012531.1
• NCBI GenBank Nucleotide #: NM_001012513.1
• Molecular Weight: 15,533 Da
• NCBI Official Full Name: gastrin-releasing peptide isoform 3 preproprotein
• NCBI Official Synonym Full Names: gastrin-releasing peptide
• NCBI Official Symbol: GRP
• NCBI Official Synonym Symbols: BN; GRP-10; proGRP; preproGRP
• NCBI Protein Information: gastrin-releasing peptide; bombesin; prepro-GRP; neuromedin C; pre-progastrin releasing peptide
• UniProt Protein Name: Gastrin-releasing peptide
• Protein Family: Gastrin-releasing peptide
• UniProt Gene Name: GRP
• UniProt Synonym Gene Names: GRP
• UniProt Entry Name: GRP_HUMAN

NCBI Description

This gene encodes a member of the bombesin-like family of gastrin-releasing peptides. Its preproprotein, following cleavage of a signal peptide, is further processed to produce either the 27 aa gastrin-releasing peptide or the 10 aa neuromedin C. These smaller peptides regulate numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation. These peptides are also likely to play a role in human cancers of the lung, colon, stomach, pancreas, breast, and prostate. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

GRP: GRP stimulates gastrin release as well as other gastrointestinal hormones. Operates as a negative feedback regulating fear and established a causal relationship between GRP- receptor gene expression, long-term potentiation, and amygdala- dependent memory for fear. Belongs to the bombesin/neuromedin-B/ranatensin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 18q21.1-q21.32

Cellular Component: extracellular space; extracellular region

Molecular Function: neuropeptide hormone activity; receptor binding

Biological Process: neuropeptide signaling pathway; signal transduction