Mouse Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) ELISA Kit | NOD2 elisa kit

Mouse Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) ELISA Kit

Cat. Num. AAA2610965

• Gene Names: NOD2; CD; ACUG; BLAU; IBD1; YAOS; BLAUS; NLRC2; NOD2B; CARD15; CLR16.3; PSORAS1
• Reactivity: Mouse
• Synonyms: Nucleotide-binding oligomerization domain-containing protein 2 (NOD2); Mouse Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) ELISA Kit; NOD2 elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 1013

• Samples: Mouse Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant No Concentration
• Assay Type: Sandwich
• Detection Range: 100 nmol/ml-1.56nmol/ml
• Sensitivity: 0.5 nmol/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOD2 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse NO monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 649572336
• NCBI GeneID: 64127
• NCBI Accession #: NP_001280486.1
• NCBI GenBank Nucleotide #: NM_001293557.1
• UniProt Accession #: Q9HC29; Q96RH5; Q96RH6; Q96RH8; E2JEQ6
• Molecular Weight: 112,530 Da
• NCBI Official Full Name: nucleotide-binding oligomerization domain-containing protein 2 isoform 2
• NCBI Official Synonym Full Names: nucleotide binding oligomerization domain containing 2
• NCBI Official Symbol: NOD2
• NCBI Official Synonym Symbols: CD; ACUG; BLAU; IBD1; YAOS; BLAUS; NLRC2; NOD2B; CARD15; CLR16.3; PSORAS1
• NCBI Protein Information: nucleotide-binding oligomerization domain-containing protein 2
• UniProt Protein Name: Nucleotide-binding oligomerization domain-containing protein 2
• Protein Family: Nucleotide-binding oligomerization domain-containing protein
• UniProt Gene Name: NOD2
• UniProt Synonym Gene Names: CARD15; IBD1

NCBI Description

This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Uniprot Description

Involved in gastrointestinal immunity. Upon stimulation by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3, INAVA and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response. Required for MDP-induced NLRP1-dependent CASP1 activation and IL1B release in macrophages (PubMed:18511561). Component of an autophagy-mediated antibacterial pathway together with ATG16L1 (PubMed:20637199). Plays also a role in sensing single-stranded RNA (ssRNA) from viruses. Interacts with mitochondrial antiviral signaling/MAVS, leading to activation of interferon regulatory factor-3/IRF3 and expression of type I interferon (PubMed:19701189).