Mouse Acetylcholinesterase (AchE) ELISA Kit | AchE elisa kit

Mouse Acetylcholinesterase (AchE) ELISA Kit

Cat. Num. AAA260553

• Gene Names: ACHE; YT; ACEE; ARACHE; N-ACHE
• Reactivity: Mouse
• Synonyms: Acetylcholinesterase (AchE); Mouse Acetylcholinesterase (AchE) ELISA Kit; AchE elisa kit

• Reactivity: Mouse
• Sequence Length: 72

• Samples: Mouse serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/mL-0.312 ng/mL
• Sensitivity: Up to 0.06 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AchE elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse AchE monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 545225
• NCBI GeneID: 43
• Molecular Weight: 58,352 Da
• NCBI Official Full Name: acetylcholinesterase, partial
• NCBI Official Synonym Full Names: acetylcholinesterase (Yt blood group)
• NCBI Official Symbol: ACHE
• NCBI Official Synonym Symbols: YT; ACEE; ARACHE; N-ACHE
• NCBI Protein Information: acetylcholinesterase; Yt blood group; apoptosis-related acetylcholinesterase
• UniProt Protein Name: Acetylcholinesterase
• Protein Family: Acetylcholinesterase
• UniProt Gene Name: ACHE
• UniProt Synonym Gene Names: AChE
• UniProt Entry Name: ACES_HUMAN

NCBI Description

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]

Uniprot Description

ACHE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Hydrolase; Lipid Metabolism - glycerophospholipid; EC 3.1.1.7

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; perinuclear region of cytoplasm; plasma membrane; extracellular region; synapse; basal lamina; nucleus; cell junction; neuromuscular junction

Molecular Function: collagen binding; serine hydrolase activity; protein binding; protein homodimerization activity; protein self-association; cholinesterase activity; hydrolase activity; beta-amyloid binding; laminin binding; acetylcholinesterase activity; acetylcholine binding

Biological Process: negative regulation of synaptic transmission, cholinergic; nervous system development; muscle development; acetylcholine catabolic process in synaptic cleft; acetylcholine catabolic process; glycerophospholipid biosynthetic process; osteoblast development; synaptic transmission; cell proliferation; synaptogenesis; positive regulation of protein secretion; amyloid precursor protein metabolic process; retina development in camera-type eye; neurotransmitter receptor biosynthetic process; phospholipid metabolic process; receptor internalization; response to wounding; phosphatidylcholine biosynthetic process; regulation of receptor recycling; DNA replication; cell adhesion; protein tetramerization; neurotransmitter biosynthetic process

Disease: Yt Blood Group Antigen