Human frizzled homolog 9 (Drosophila) ELISA Kit | FZD9 elisa kit

Human Frizzled-9, FZD9 ELISA Kit

Cat. Num. AAA2602762

• Gene Names: FZD9; FZD3; CD349
• Reactivity: Human
• Synonyms: frizzled homolog 9 (Drosophila); Human Frizzled-9; FZD9 ELISA Kit; FZD9 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.

• Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant FZD9 concentration
• Assay Type: Sandwich
• Detection Range: 50 ng/ml-0.78 ng/ml
• Sensitivity: 0.1 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FZD9 elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human FZD9 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4503835
• NCBI GeneID: 8326
• NCBI Accession #: NP_003499.1
• NCBI GenBank Nucleotide #: NM_003508.2
• UniProt Accession #: O00144
• Molecular Weight: 64,466 Da
• NCBI Official Full Name: frizzled-9
• NCBI Official Synonym Full Names: frizzled class receptor 9
• NCBI Official Symbol: FZD9
• NCBI Official Synonym Symbols: FZD3; CD349
• NCBI Protein Information: frizzled-9; fz-9; fzE6; hFz9; frizzled homolog 9; frizzled family receptor 9; frizzled 9, seven transmembrane spanning receptor
• UniProt Protein Name: Frizzled-9
• Protein Family: Frizzled
• UniProt Gene Name: FZD9
• UniProt Synonym Gene Names: FZD3; Fz-9; hFz9
• UniProt Entry Name: FZD9_HUMAN

NCBI Description

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD9: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, Fz/Smo family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: filopodium membrane; cell surface; perinuclear region of cytoplasm; cytoplasm; plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein homodimerization activity; protein heterodimerization activity

Biological Process: G-protein coupled receptor protein signaling pathway; nervous system development; learning and/or memory; B cell differentiation; neuroblast proliferation; Wnt receptor signaling pathway through beta-catenin