Rat von Willebrand Factor cleaving protease (ADAMTS-13 / vWF-cp) ELISA Kit | ADAMTS-13 / vWF-cp elisa kit

Rat von Willebrand Factor cleaving protease (ADAMTS-13 / vWF-cp) ELISA Kit

Cat. Num. AAA2602135

• Gene Names: ADAMTS13; VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
• Reactivity: Rat
• Synonyms: von Willebrand Factor cleaving protease (ADAMTS-13 / vWF-cp); Rat von Willebrand Factor cleaving protease (ADAMTS-13 / vWF-cp) ELISA Kit; ADAMTS-13 / vWF-cp elisa kit

• Reactivity: Rat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 1427

• Samples: Rat serum, plasma or cell culture supernatant
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/mL
• Intra-assay Precision: ? 8%
• Inter-assay Precision: ? 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAMTS-13 / vWF-cp elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat ADAMTS-13/vWF-c monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 16306598
• NCBI GeneID: 11093
• Molecular Weight: 39,864 Da
• NCBI Official Full Name: von Willebrand factor-cleaving protease
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 13
• NCBI Official Symbol: ADAMTS13
• NCBI Official Synonym Symbols: VWFCP; C9orf8; vWF-CP; ADAM-TS13; ADAMTS-13
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 13; vWF-cleaving protease; von Willebrand factor-cleaving protease; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 13
• UniProt Gene Name: ADAMTS13
• UniProt Synonym Gene Names: C9orf8; UNQ6102/PRO20085; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP; vWF-cleaving protease
• UniProt Entry Name: ATS13_HUMAN

NCBI Description

This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

ADAMTS13: Cleaves the vWF multimers in plasma into smaller forms. Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP); also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Secreted; Calcium-binding; Motility/polarity/chemotaxis; Secreted, signal peptide; EC 3.4.24.87; Extracellular matrix

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; endoplasmic reticulum lumen

Molecular Function: integrin binding; protein binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding

Biological Process: integrin-mediated signaling pathway; protein amino acid O-linked glycosylation; platelet activation; cellular protein metabolic process; glycoprotein metabolic process; cell-matrix adhesion; peptide catabolic process; response to toxin; protein processing; proteolysis; post-translational protein modification

Disease: Thrombotic Thrombocytopenic Purpura, Congenital